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I have bought on September a vial of Anti-Survivin antibody [60.11] – ab93274 lot number GR88156 -1 (order number 1160799).
I have always used this clone for my experiments on human hematopoietic cell lines and I have had any problems.
I tested this new stock and I obtained a blot blank.
I have loaded from 10µg up to 90µg of cell lysate on SDS-PAGE gel and have blotted the membrane with a dilution from 1:200 up to 1:1000 but I did not obtained any staining.
How I can solve this problem?
Do you have any suggestion?
Thank you for your attention on this matter.
Asked on Oct 12 2012
Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.
Answered on Oct 12 2012