Question (64055) | Anti-Survivin antibody [60.11] (ab93274)

Go to datasheet (ab93274)

Question

you will find the answers to your questions:

The image is totally clean so could I double check? I blot the same membrane for anti-p21 and I obtained a band to the correct size

have you used 400 cells/ well or 50- 90ug protein/ lane? The image that I have attached to the e-mail represent different µg of whole lysate for lane. In another gel I tried to load 400.000 cell/line as protocol that I used in my lab.
- Did you observe same clean membrane after trying 3 times? yes
- Is the secondary antibody fine? Have you tried "loading control" with the same lysates? the secondary antibody is correct and I load the same lysate on another gels and I blotted the membrane with other antibodies.
- Is it true no protease inhibitors were added? Could you try after adding a PI cocktail? the Laemmli buffer does nor require the addition of protease inhibitors.

I would like to test another batch of this antibody and if I have the same results I buy another antibody.

Thank you for your attention on this matter.

Best regards

Answer

We unfortunately do not have new batch available yet. Would like to try another vial from same batch?

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