Question (67779) | Anti-Survivin antibody [60.11] (ab93274)

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Question

I have bought on September a vial of Anti-Survivin antibody [60.11] – ab93274 lot number GR88156 -1 (order number 1160799).
I have always used this clone for my experiments on human hematopoietic cell lines and I have had any problems.
I tested this new stock and I obtained a blot blank.
I have loaded from 10µg up to 90µg of cell lysate on SDS-PAGE gel and have blotted the membrane with a dilution from 1:200 up to 1:1000 but I did not obtained any staining.
How I can solve this problem?
Do you have any suggestion?
Thank you for your attention on this matter.

Answer

Thank for your email. Apologies for delay.

We have no other complaints regarding the stability of this antibody so it seems like this is one off case.

I would be happy to send you a replacement vial if you are interested. Please confirm.

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