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I have bought on September a vial of Anti-Survivin antibody [60.11] – ab93274 lot number GR88156 -1 (order number 1160799).
I have always used this clone for my experiments on human hematopoietic cell lines and I have had any problems.
I tested this new stock and I obtained a blot blank.
I have loaded from 10µg up to 90µg of cell lysate on SDS-PAGE gel and have blotted the membrane with a dilution from 1:200 up to 1:1000 but I did not obtained any staining.
How I can solve this problem?
Do you have any suggestion?
Thank you for your attention on this matter.
Asked on Oct 18 2012