Anti-Survivin antibody (ab27468)

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Overview

  • Product name

    Anti-Survivin antibody
    See all Survivin primary antibodies

  • Description

    Goat polyclonal to Survivin

  • Host species

    Goat

  • Tested applications

    Suitable for: Sandwich ELISA, ELISA, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog

  • Immunogen

    Synthetic peptide:

    (C)VRRAIEQLAAMD

    , corresponding to C terminal amino acids 131-142 of Human Survivin

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human T cell line MOLT4 lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab27468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP2880Y] to Survivin (ab76424).

For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab76424 as Detection.
ELISA 1/16000.
WB Use a concentration of 0.01 - 0.5 µg/ml. Detects a band of approximately 18 kDa.

Target

  • Function

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

  • Tissue specificity

    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

  • Sequence similarities

    Belongs to the IAP family.
    Contains 1 BIR repeat.

  • Developmental stage

    Expression is cell cycle-dependent and peaks at mitosis.

  • Domain

    The BIR repeat is necessary and sufficient for HBXIP binding.

  • Post-translational
    modifications

    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.

  • Cellular localization

    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt

  • Database links

    • Alternative names

      • API4 antibody
      • Apoptosis inhibitor 4 antibody
      • Apoptosis inhibitor survivin antibody
      • Apoptosis inhibitor4 antibody
      • Baculoviral IAP repeat containing 5 antibody
      • Baculoviral IAP repeat containing protein 5 antibody
      • Baculoviral IAP repeat-containing protein 5 antibody
      • BIRC 5 antibody
      • BIRC5 antibody
      • BIRC5_HUMAN antibody
      • EPR 1 antibody
      • IAP4 antibody
      • Survivin variant 3 alpha antibody
      • SVV antibody
      • TIAP antibody
      see all

    Images

    References

    This product has been referenced in:

    • Zhang S  et al. Targeted inhibition of survivin with YM155 promotes apoptosis of hypoxic human pulmonary arterial smooth muscle cells via the upregulation of voltage-dependent K? channels. Mol Med Rep 13:3415-22 (2016). ICC . Read more (PubMed: 26957114) »
    See 1 Publication for this product

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