Anti-Survivin antibody (ab27468)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Survivin antibody
See all Survivin primary antibodies -
DescriptionGoat polyclonal to Survivin
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Host speciesGoat
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Tested applicationsSuitable for: Sandwich ELISA, ELISA, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Dog -
Immunogen
Synthetic peptide:
(C)VRRAIEQLAAMD
, corresponding to C terminal amino acids 131-142 of Human Survivin -
Positive control
- Human T cell line MOLT4 lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab27468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA | Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [EP2880Y] to Survivin (ab76424). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab76424 as Detection. |
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ELISA | 1/16000. | |
WB | Use a concentration of 0.01 - 0.5 µg/ml. Detects a band of approximately 18 kDa. |
Target
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FunctionComponent of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
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Tissue specificityExpressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
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Sequence similaritiesBelongs to the IAP family.
Contains 1 BIR repeat. -
Developmental stageExpression is cell cycle-dependent and peaks at mitosis.
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DomainThe BIR repeat is necessary and sufficient for HBXIP binding.
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Post-translational
modificationsUbiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
Cellular localizationCytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
- Information by UniProt
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Database links
- Entrez Gene: 442936 Dog
- Entrez Gene: 332 Human
- Omim: 603352 Human
- SwissProt: Q8I009 Dog
- SwissProt: O15392 Human
- Unigene: 514527 Human
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Alternative names
- API4 antibody
- Apoptosis inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
Images
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Standard curve for Survivin (Analyte: ab87202); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab27468 at 1µg/ml and Detector Antibody abRabbit monoclonal [EP2880Y] to Survivin (ab76424) at 0.5µg/ml. Concentration of Rabbit monoclonal [EP2880Y] to Survivin (ab76424) may vary from lot to lot; please use this curve as guideline.
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Anti-Survivin antibody (ab27468) at 0.01 µg/ml + Molt-4 lysate at 35 µg
Observed band size: 18 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhang S et al. Targeted inhibition of survivin with YM155 promotes apoptosis of hypoxic human pulmonary arterial smooth muscle cells via the upregulation of voltage-dependent K? channels. Mol Med Rep 13:3415-22 (2016). ICC . Read more (PubMed: 26957114) »