Overview

  • Product name
  • Description
    Rabbit polyclonal to Survivin
  • Host species
    Rabbit
  • Specificity
    Specific for survivin.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-P, ELISA, Flow Cyt, IP, RIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Cat, Dog, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Survivin .
    Database link: O15392

  • Positive control
    • WB: Jurkat whole cell lysate (ab7899), HeLa whole cell lysate (ab150035). ICC: HeLa cells. IHC-P: Human ovarian carcinoma, rectal cancer and colon carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250. See Abreview by William Moore; fix with formaldehyde.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution.
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration. Recommended to use at 5-7µg/ml.
RIP Use at an assay dependent concentration. PubMed: 19542185

Target

  • Function
    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificity
    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similarities
    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stage
    Expression is cell cycle-dependent and peaks at mitosis.
  • Domain
    The BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications
    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localization
    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • API4 antibody
    • Apoptosis inhibitor 4 antibody
    • Apoptosis inhibitor survivin antibody
    • Apoptosis inhibitor4 antibody
    • Baculoviral IAP repeat containing 5 antibody
    • Baculoviral IAP repeat containing protein 5 antibody
    • Baculoviral IAP repeat-containing protein 5 antibody
    • BIRC 5 antibody
    • BIRC5 antibody
    • BIRC5_HUMAN antibody
    • EPR 1 antibody
    • IAP4 antibody
    • Survivin variant 3 alpha antibody
    • SVV antibody
    • TIAP antibody
    see all

Images

  • All lanes : Anti-Survivin antibody (ab469) at 1 µg/ml

    Lane 1 : HeLa Nuclear
    Lane 2 : HeLa whole cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : HEK293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 16 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

  • Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 µg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

  • ab469 staining Survivin from Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.

    See Abreview

  • HeLa cells (ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK. 

  • ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.

    This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.

    See Abreview

  • ab469 immunoprecipitating Survivin from HeLa cell lysate. HeLa cells were lysed after colcemid block (ON) and immunoprecipitated with ab469 at a 1/1000 dilution. HeLa cells clear lysate (Lane 1) as well as the bound material (Lane 2) were loaded on a 15 % acrylamide gel. An HRP conjugated Donkey Anti-rabbit IgG was used as the secondary antibody.

    This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 12 April 2006.

    See Abreview

  • Anti-Survivin antibody (ab469) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 16 kDa


    Exposure time: 1 minute
  • Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma tissue stained for Survivin using ab469 at 1/500 dilution in immunohistochemical analysis.

    See Abreview

References

This product has been referenced in:
See all 80 Publications for this product

Customer reviews and Q&As

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1-10 of 10 Abreviews

Application
Western blot
Sample
Mouse Cell lysate - whole cell (endothelial)
Gel Running Conditions
Reduced Denaturing (14)
Loading amount
10 µg
Specification
endothelial
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 04 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Human colon carcinoma cells)
Loading amount
75 µg
Specification
Human colon carcinoma cells
Gel Running Conditions
Non-reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Eleni Petsalaki

Verified customer

Submitted Oct 08 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (ovarian cancer)
Specification
ovarian cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH 6.0, 100C, 20 min
Permeabilization
No

Abcam user community

Verified customer

Submitted Dec 17 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
50 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 26 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon-Carcinoma)
Specification
Colon-Carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated

Mr. Rudolf Jung

Verified customer

Submitted Feb 28 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (macrophage)
Specification
macrophage
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Abcam user community

Verified customer

Submitted Nov 21 2006

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovarian carcinoma effusions)
Specification
Ovarian carcinoma effusions
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Other as blocking agent for 5 minute(s) · Concentration: 0.03%

Dr. Ben Davidson

Verified customer

Submitted Jul 18 2006

Application
Immunoprecipitation
Sample
Human Cell lysate - other (HeLa)
Total protein in input
25 µg
Specification
HeLa
Immuno-precipitation step
Protein A

Dr. Sandrine Ruchaud

Verified customer

Submitted Apr 12 2006

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Dr. Sandrine Ruchaud

Verified customer

Submitted Apr 03 2006

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%

Dr. Sandrine Ruchaud

Verified customer

Submitted Mar 30 2006

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