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    survivin-antibody-ab469.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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Anti-Survivin antibody (ab469)

  • Datasheet
  • SDS
Reviews (10)Q&A (21)References (105)

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Western blot - Anti-Survivin antibody (ab469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
  • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
  • Western blot - Anti-Survivin antibody (ab469)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)

Key features and details

  • Rabbit polyclonal to Survivin
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Survivin antibody [EP2880Y] (ab76424)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Survivin antibody
    See all Survivin primary antibodies
  • Description

    Rabbit polyclonal to Survivin
  • Host species

    Rabbit
  • Specificity

    Specific for survivin.
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Survivin .
    Database link: O15392

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.876% Sodium chloride, 99% Tris glycine
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Survivin protein (ab87202)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
1/250. See Abreview by William Moore; fix with formaldehyde.
WB (4)
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution.
IHC-P (3)
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Notes
ICC/IF
1/250. See Abreview by William Moore; fix with formaldehyde.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Found to work at 1/5000 dilution.
IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Function

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificity

    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stage

    Expression is cell cycle-dependent and peaks at mitosis.
  • Domain

    The BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications

    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Target information above from: UniProt accession O15392 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 332 Human
    • Omim: 603352 Human
    • SwissProt: O15392 Human
    • Unigene: 514527 Human
    • Alternative names

      • API4 antibody
      • Apoptosis inhibitor 4 antibody
      • Apoptosis inhibitor survivin antibody
      • Apoptosis inhibitor4 antibody
      • Baculoviral IAP repeat containing 5 antibody
      • Baculoviral IAP repeat containing protein 5 antibody
      • Baculoviral IAP repeat-containing protein 5 antibody
      • BIRC 5 antibody
      • BIRC5 antibody
      • BIRC5_HUMAN antibody
      • EPR 1 antibody
      • IAP4 antibody
      • Survivin variant 3 alpha antibody
      • SVV antibody
      • TIAP antibody
      see all

    Images

    • Western blot - Anti-Survivin antibody (ab469)
      Western blot - Anti-Survivin antibody (ab469)
      All lanes : Anti-Survivin antibody (ab469) at 1 µg/ml

      Lane 1 : HeLa Nuclear
      Lane 2 : HeLa whole cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : Jurkat cell lysate
      Lane 5 : HEK293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution

      Performed under reducing conditions.

      Predicted band size: 16 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?
      Additional bands at: 37 kDa, 50 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)

      Paraffin-embedded human rectal cancer tissue stained for Survivin using ab469 at 0.5 µg/ml in immunohistochemical analysis, using DAB with hematoxylin counterstain.

    • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
      Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)

      HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Survivin (green) using ab469 at 1/10 dilution in ICC/IF. An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)This image is courtesy of an Abreview submitted by Dr Ben Davidson

      ab469 staining Survivin from Human Ovarian carcionoma tumour tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed (Citrate buffer pH=6, microwave oven) and the tissue was then formaldehyde fixed and blocked (Hydrogen peroxide 0.03%). An HRP conjugated goat anti-rabbit was used as the secondary antibody.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
      Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)This image is courtesy of William Moore

      HeLa cells (ab150035) in prometaphase, metaphase and anaphase stained with anti-Survivin (green), anti-tubulin (red) and DAPI (blue). These images were kindly supplied as part of the review submitted by William Moore, University of Dundee, UK. 

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)
      Immunocytochemistry/ Immunofluorescence - Anti-Survivin antibody (ab469)

      ab469 at a 1/400 dilution staining HeLa cells by Immunocytochemistry. The antibody was incubated with the cells for 1 hour and then was detected using a Texas Red conjugated Goat anti-rabbit antibody.

      This image is courtesy of an Abreview by Sandrine Ruchaud submitted on 30 March 2006.

      See Abreview

    • Western blot - Anti-Survivin antibody (ab469)
      Western blot - Anti-Survivin antibody (ab469)
      Anti-Survivin antibody (ab469) at 1 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

      Developed using the ECL technique.

      Predicted band size: 16 kDa


      Exposure time: 1 minute
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody (ab469)This image is courtesy of an Abreview submitted by Mr. Rudolf Jung.
      Paraformaldehyde-fixed, paraffin-embedded human colon carcinoma tissue stained for Survivin using ab469 at 1/500 dilution in immunohistochemical analysis.

      See Abreview

    Protocols

    • Recommended protocols with ab469

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (105)

    Publishing research using ab469? Please let us know so that we can cite the reference in this datasheet.

    ab469 has been referenced in 105 publications.

    • Li Q  et al. Silencing of synaptotagmin 13 inhibits tumor growth through suppressing proliferation and promoting apoptosis of colorectal cancer cells. Int J Mol Med 45:234-244 (2020). PubMed: 31939613
    • Wang Q  et al. Identification of KIF15 as a potential therapeutic target and prognostic factor for glioma. Oncol Rep 43:1035-1044 (2020). PubMed: 32323839
    • Chehelgerdi M & Doosti A Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine. J Nanobiotechnology 18:63 (2020). PubMed: 32316990
    • Wang J  et al. miR-378 in combination with ultrasonic irradiation and SonoVue microbubbles transfection inhibits hepatoma cell growth. Mol Med Rep 21:2493-2501 (2020). PubMed: 32236628
    • Tian Y  et al. Combined Snail and E-cadherin Predicts Overall Survival of Cervical Carcinoma Patients: Comparison Among Various Epithelial-Mesenchymal Transition Proteins. Front Mol Biosci 7:22 (2020). PubMed: 32185181
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

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    1-10 of 10 Abreviews

    Western blot abreview for Anti-Survivin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (endothelial)
    Gel Running Conditions
    Reduced Denaturing (14)
    Loading amount
    10 µg
    Specification
    endothelial
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 04 2017

    Western blot abreview for Anti-Survivin antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human colon carcinoma cells)
    Loading amount
    75 µg
    Specification
    Human colon carcinoma cells
    Gel Running Conditions
    Non-reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Eleni Petsalaki

    Verified customer

    Submitted Oct 08 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Survivin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (ovarian cancer)
    Specification
    ovarian cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, pH 6.0, 100C, 20 min
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 17 2010

    Western blot abreview for Anti-Survivin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    50 µg
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 26 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Survivin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon-Carcinoma)
    Specification
    Colon-Carcinoma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Read More

    Mr. Rudolf Jung

    Verified customer

    Submitted Feb 28 2007

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Survivin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (macrophage)
    Specification
    macrophage
    Fixative
    Formaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 21 2006

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Survivin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Ovarian carcinoma effusions)
    Specification
    Ovarian carcinoma effusions
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Other as blocking agent for 5 minute(s) · Concentration: 0.03%
    Read More

    Dr. Ben Davidson

    Verified customer

    Submitted Jul 18 2006

    Immunoprecipitation abreview for Anti-Survivin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - other (HeLa)
    Total protein in input
    25 µg
    Specification
    HeLa
    Immuno-precipitation step
    Protein A
    Read More

    Dr. Sandrine Ruchaud

    Verified customer

    Submitted Apr 12 2006

    Western blot abreview for Anti-Survivin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    20 µg
    Specification
    HeLa cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Dr. Sandrine Ruchaud

    Verified customer

    Submitted Apr 03 2006

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Survivin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Read More

    Dr. Sandrine Ruchaud

    Verified customer

    Submitted Mar 30 2006

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