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I am overexpressing human survivin delta Ex3 in a cell line. Shouldn’t this antibody (ab76424) detect this form since it corresponds to the N terminus of survivin? I have tried using the survivin DEx3 antibody (ab3731) but can’t detect it with that antibody either. I appreciate any feedback you can give me.
Asked on Oct 20 2011
Thank you for contacting Abcam regarding ab76424 and ab3731. I am sorry that you have been experiencing difficulties getting these antibodies to work in WB. I am following up with the lab regarding reactivity of ab76424 with Delta Ex3. Regarding ab3731, one point to note is that this antibody only works well in Western blotting on cell lysates overexpressing DEx3(see PMID 16847456), and it first requires IP with another antibody, before blotting with this antibody. It has been previously used with an IP performed from 5 million cells overexpressing DEx3. If you have not performed an IP, the antibody will not likely detect the protein in WB. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.
Answered on Oct 20 2011