Western blot abreview for Anti-Survivin antibody [EP2880Y]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial cell)
Loading amount
30 µg
Specification
Epithelial cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk

Secondary antibody

Name
Non-Abcam antibody was used: Peroxidase-conjugated AffiniPure Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~16.5 kDa Non-specific: 15 kDa

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Submitted Jan 06 2010

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