Western blot abreview for Anti-Survivin antibody [EP2880Y]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Ovarian cancer cells OVCAR-3)
Loading amount
40 µg
Specification
Ovarian cancer cells OVCAR-3
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/5000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-linked Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 16 kDa

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Submitted Sep 21 2011

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