Western blot abreview for Anti-Survivin antibody [EP2880Y]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma Initiating Cells (Primary cells))
Loading amount
30 µg
Specification
Glioblastoma Initiating Cells (Primary cells)
Gel Running Conditions
Reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk 1% in TBS Tween 0.1%

Secondary antibody

Name
Non-Abcam antibody was used: Bio-Rad Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 17 kDa

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Submitted Nov 16 2011

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