Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Survivin antibody [EP2880Y]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Sample
Human Tissue sections (human glioblastoma xenograft in nude mice)
Specification
human glioblastoma xenograft in nude mice
Permeabilization
Yes - Triton 0.025%
Fixative
Alcool-Formol-Acetate (AFA)

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Dako antibody diluent
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Dako EnVision+ System-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

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Submitted Jul 26 2013

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