Immunocytochemistry abreview for Anti-Survivin antibody [EP2880Y]

Excellent
Abreviews
Application
Immunocytochemistry
Sample
Human Cell (Human squamous cell carcinoma cell line H520)
Permeabilization
Yes - 0.25% Triton X-100/PBS
Specification
Human squamous cell carcinoma cell line H520
Blocking step
RotiBlock as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: RotiBlock
Dilution
1/300

Secondary antibody

Dilution
1/1200
Name
Non-Abcam antibody was used: LiCor anti rabbit 800CW IRDye
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Additional data

Additional Notes
Works excellent in In-Cell Western/In-Cell ELISA assay

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Submitted Mar 31 2017

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