Immunocytochemistry/ Immunofluorescence abreview for Anti-Survivin antibody [EP2880Y]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/500

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Asynchronous HeLa cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, the antibody produces a strong kinetochore-associated staining pattern in early mitotic cells that following entry into anaphase, produces a mid-body staining pattern. MeOH fixed samples were also evaluated and gave a similar staining pattern.

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 24 2017

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