Asynchronous HeLa cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, the antibody produces a strong kinetochore-associated staining pattern in early mitotic cells that following entry into anaphase, produces a mid-body staining pattern. MeOH fixed samples were also evaluated and gave a similar staining pattern.
Dr. Kirk Mcmanus
Submitted Aug 24 2017
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