Recombinant Anti-Survivin antibody [EPR17358] (ab182132)
![Western blot - Anti-Survivin antibody [EPR17358] (ab182132)](https://www.abcam.com/ps/products/182/ab182132/Images/ab182132-234768-anti-survivin-antibody-epr17358-western-blot.jpg "Western blot - Anti-Survivin antibody [EPR17358] (ab182132)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17358] to Survivin
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Survivin antibody [EPR17358]
See all Survivin primary antibodies -
Description
Rabbit monoclonal [EPR17358] to Survivin -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Neuro-2a, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse colon and testis lysates. IHC-P: Mouse spleen and testis tissues. IP: F9 whole cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17358 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/100.
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
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| Notes |
|---|
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IP
1/100. |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. -
Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. -
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat. -
Developmental stage
Expression is cell cycle-dependent and peaks at mitosis. -
Domain
The BIR repeat is necessary and sufficient for HBXIP binding. -
Post-translational
modificationsUbiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
Cellular localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
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Database links
- Entrez Gene: 11799 Mouse
- Entrez Gene: 64041 Rat
- SwissProt: O70201 Mouse
- SwissProt: Q9JHY7 Rat
- Unigene: 8552 Mouse
- Unigene: 54471 Rat
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Alternative names
- API4 antibody
- Apoptosis inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
Images
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Western blot - Anti-Survivin antibody [EPR17358] (ab182132)All lanes : Anti-Survivin antibody [EPR17358] (ab182132) at 1/1000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates
Lane 2 : Mouse colon lysates
Lane 3 : Mouse testis lysates
Lane 4 : C6 (Rat glial tumor cells) whole cell lysates
Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)](https://www.abcam.com/ps/products/182/ab182132/Images/ab182132-234765-anti-survivin-antibody-epr17358-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on mouse spleen is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)](https://www.abcam.com/ps/products/182/ab182132/Images/ab182132-234767-anti-survivin-antibody-epr17358-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on spermatogenic cells of mouse testis is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)](https://www.abcam.com/ps/products/182/ab182132/Images/ab182132-234760-anti-survivin-antibody-epr17358-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)Survivin was immunoprecipitated from 1mg of F9 (Mouse embyro testicular cancer cell line) whole cell extract with ab182132 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab182132 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: F9 whole cell extract. Lane 2: PBS instead of F9 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-Survivin antibody [EPR17358] (ab182132)](https://www.abcam.com/ps/products/182/ab182132/Images/ab182132-8-benefits-of-recombinant-antibodies.png)
Anti-Survivin antibody [EPR17358] (ab182132)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (5)
ab182132 has been referenced in 5 publications.
- Wang M et al. The Ectopic Expression of SurvivinT34A and FilC Can Enhance the Oncolytic Effects of Vaccinia Virus in Murine Gastric Cancer. Onco Targets Ther 13:1011-1025 (2020). PubMed: 32099404
- Mika A et al. Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma. J Clin Med 8:N/A (2019). PubMed: 30909651
- Deng S et al. HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30832202
- Yao L & Zhang Z The reversal of MRP1 expression induced by low-frequency and low-intensity ultrasound and curcumin mediated by VEGF in brain glioma. Onco Targets Ther 12:3581-3593 (2019). PubMed: 31190861
- He L & Gu K Tanshinone IIA regulates colorectal cancer apoptosis via attenuation of Parkin-mediated mitophagy by suppressing AMPK/Skp2 pathways. Mol Med Rep 18:1692-1703 (2018). PubMed: 29845197
