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  1. Link

    survivin-antibody-epr17358-ab182132.pdf

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Cell Biology Apoptosis Intracellular Survivin / IAPs
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RecombinantRabMAb

Recombinant Anti-Survivin antibody [EPR17358] (ab182132)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (5)

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Western blot - Anti-Survivin antibody [EPR17358] (ab182132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
  • Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)
  • Anti-Survivin antibody [EPR17358] (ab182132)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17358] to Survivin
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-Survivin antibody [EPR17358]
    See all Survivin primary antibodies
  • Description

    Rabbit monoclonal [EPR17358] to Survivin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Neuro-2a, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse colon and testis lysates. IHC-P: Mouse spleen and testis tissues. IP: F9 whole cell extract.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17358
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Survivin / IAPs
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • IAPs
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • IAPs

Associated products

  • Alternative Versions

    • HRP Anti-Survivin antibody [EPR17358] (ab209466)
    • Anti-Survivin antibody [EPR17358] - BSA and Azide free (ab250577)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Survivin protein (ab87202)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Notes
IP
1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • Tissue specificity

    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • Sequence similarities

    Belongs to the IAP family.
    Contains 1 BIR repeat.
  • Developmental stage

    Expression is cell cycle-dependent and peaks at mitosis.
  • Domain

    The BIR repeat is necessary and sufficient for HBXIP binding.
  • Post-translational
    modifications

    Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • Cellular localization

    Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Target information above from: UniProt accession O15392 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 11799 Mouse
    • Entrez Gene: 64041 Rat
    • SwissProt: O70201 Mouse
    • SwissProt: Q9JHY7 Rat
    • Unigene: 8552 Mouse
    • Unigene: 54471 Rat
    • Alternative names

      • API4 antibody
      • Apoptosis inhibitor 4 antibody
      • Apoptosis inhibitor survivin antibody
      • Apoptosis inhibitor4 antibody
      • Baculoviral IAP repeat containing 5 antibody
      • Baculoviral IAP repeat containing protein 5 antibody
      • Baculoviral IAP repeat-containing protein 5 antibody
      • BIRC 5 antibody
      • BIRC5 antibody
      • BIRC5_HUMAN antibody
      • EPR 1 antibody
      • IAP4 antibody
      • Survivin variant 3 alpha antibody
      • SVV antibody
      • TIAP antibody
      see all

    Images

    • Western blot - Anti-Survivin antibody [EPR17358] (ab182132)
      Western blot - Anti-Survivin antibody [EPR17358] (ab182132)
      All lanes : Anti-Survivin antibody [EPR17358] (ab182132) at 1/1000 dilution

      Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates
      Lane 2 : Mouse colon lysates
      Lane 3 : Mouse testis lysates
      Lane 4 : C6 (Rat glial tumor cells) whole cell lysates
      Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
      Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
      Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 16 kDa
      Observed band size: 16 kDa



      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on mouse spleen is observed. Counter stained with Hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on spermatogenic cells of mouse testis is observed. Counter stained with Hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)
      Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)

      Survivin was immunoprecipitated from 1mg of F9 (Mouse embyro testicular cancer cell line) whole cell extract with ab182132 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab182132 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: F9 whole cell extract. Lane 2: PBS instead of F9 whole cell extract.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-Survivin antibody [EPR17358] (ab182132)
      Anti-Survivin antibody [EPR17358] (ab182132)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (5)

    Publishing research using ab182132? Please let us know so that we can cite the reference in this datasheet.

    ab182132 has been referenced in 5 publications.

    • Wang M  et al. The Ectopic Expression of SurvivinT34A and FilC Can Enhance the Oncolytic Effects of Vaccinia Virus in Murine Gastric Cancer. Onco Targets Ther 13:1011-1025 (2020). PubMed: 32099404
    • Mika A  et al. Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma. J Clin Med 8:N/A (2019). PubMed: 30909651
    • Deng S  et al. HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30832202
    • Yao L & Zhang Z The reversal of MRP1 expression induced by low-frequency and low-intensity ultrasound and curcumin mediated by VEGF in brain glioma. Onco Targets Ther 12:3581-3593 (2019). PubMed: 31190861
    • He L & Gu K Tanshinone IIA regulates colorectal cancer apoptosis via attenuation of Parkin-mediated mitophagy by suppressing AMPK/Skp2 pathways. Mol Med Rep 18:1692-1703 (2018). PubMed: 29845197

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Survivin antibody [EPR17358]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    DR. Kirk Mcmanus

    Verified customer

    Submitted Jan 15 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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