RecombinantRabMAb

Recombinant Anti-Survivin antibody [EPR17358] (ab182132)

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Western blot - Anti-Survivin antibody [EPR17358] (ab182132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
  • Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)
  • Anti-Survivin antibody [EPR17358] (ab182132)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17358] to Survivin
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free HRP

Overview

  • Product name

    Anti-Survivin antibody [EPR17358]
    See all Survivin primary antibodies

  • Description

    Rabbit monoclonal [EPR17358] to Survivin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Neuro-2a, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse colon and testis lysates. IHC-P: Mouse spleen and testis tissues. IP: F9 whole cell extract.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Notes
IP
1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

    • Tissue specificity

      Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

    • Sequence similarities

      Belongs to the IAP family.
      Contains 1 BIR repeat.

    • Developmental stage

      Expression is cell cycle-dependent and peaks at mitosis.

    • Domain

      The BIR repeat is necessary and sufficient for HBXIP binding.

    • Post-translational
      modifications

      Ubiquitination is required for centrosomal targeting.
      In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.

    • Cellular localization

      Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
    • Information by UniProt

    • Database links

      • Alternative names

        • API4 antibody
        • Apoptosis inhibitor 4 antibody
        • Apoptosis inhibitor survivin antibody
        • Apoptosis inhibitor4 antibody
        • Baculoviral IAP repeat containing 5 antibody
        • Baculoviral IAP repeat containing protein 5 antibody
        • Baculoviral IAP repeat-containing protein 5 antibody
        • BIRC 5 antibody
        • BIRC5 antibody
        • BIRC5_HUMAN antibody
        • EPR 1 antibody
        • IAP4 antibody
        • Survivin variant 3 alpha antibody
        • SVV antibody
        • TIAP antibody
        see all

      Images

      • Western blot - Anti-Survivin antibody [EPR17358] (ab182132)
        Western blot - Anti-Survivin antibody [EPR17358] (ab182132)
        All lanes : Anti-Survivin antibody [EPR17358] (ab182132) at 1/1000 dilution

        Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates
        Lane 2 : Mouse colon lysates
        Lane 3 : Mouse testis lysates
        Lane 4 : C6 (Rat glial tumor cells) whole cell lysates
        Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
        Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
        Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

        Predicted band size: 16 kDa
        Observed band size: 16 kDa



        Blocking/Dilution buffer: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)

        Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on mouse spleen is observed. Counter stained with Hematoxylin.

        Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR17358] (ab182132)

        Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Survivin with ab182132 at 1/500 dilution followed by Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution. Nuclear staining on spermatogenic cells of mouse testis is observed. Counter stained with Hematoxylin.

        Negative control: Using PBS instead of primary antibody, secondary antibody is Anti-Rabbit HRP (ab97051) secondary at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)
        Immunoprecipitation - Anti-Survivin antibody [EPR17358] (ab182132)

        Survivin was immunoprecipitated from 1mg of F9 (Mouse embyro testicular cancer cell line) whole cell extract with ab182132 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab182132 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: F9 whole cell extract. Lane 2: PBS instead of F9 whole cell extract.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      • Anti-Survivin antibody [EPR17358] (ab182132)
        Anti-Survivin antibody [EPR17358] (ab182132)

      References (5)

      Publishing research using ab182132? Please let us know so that we can cite the reference in this datasheet.

      ab182132 has been referenced in 5 publications.

      • Wang M  et al. The Ectopic Expression of SurvivinT34A and FilC Can Enhance the Oncolytic Effects of Vaccinia Virus in Murine Gastric Cancer. Onco Targets Ther 13:1011-1025 (2020). PubMed: 32099404
      • Mika A  et al. Epigenetic Changes at the Birc5 Promoter Induced by YM155 in Synovial Sarcoma. J Clin Med 8:N/A (2019). PubMed: 30909651
      • Deng S  et al. HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30832202
      • Yao L & Zhang Z The reversal of MRP1 expression induced by low-frequency and low-intensity ultrasound and curcumin mediated by VEGF in brain glioma. Onco Targets Ther 12:3581-3593 (2019). PubMed: 31190861
      • He L & Gu K Tanshinone IIA regulates colorectal cancer apoptosis via attenuation of Parkin-mediated mitophagy by suppressing AMPK/Skp2 pathways. Mol Med Rep 18:1692-1703 (2018). PubMed: 29845197

      Customer reviews and Q&As

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      Immunocytochemistry/ Immunofluorescence abreview for Anti-Survivin antibody [EPR17358]

       Poor
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HeLa)
      Permeabilization
      Yes - 0.5% Triton X-100
      Specification
      HeLa
      Fixative
      Paraformaldehyde
      Read More

      Dr. Kirk Mcmanus

      Verified customer

      Submitted Jan 15 2019

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