Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
Asynchronous HeLa were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, the antibody produced a strong kinetochore-assocaited labeling pattern in early mitotic cells before transitioning to the mid-body in late mitotic cells. MeOH fixed samples were also evaluated and produced a similar, albeit weaker labeling pattern.
Dr. Kirk Mcmanus
Verified customer
Submitted Nov 03 2018