Overview

  • Product name

    Anti-Survivin antibody [EPR2675]
    See all Survivin primary antibodies
  • Description

    Rabbit monoclonal [EPR2675] to Survivin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Survivin aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human cervical carcinoma tissue; HeLa and MOLT4 cell lysates.
  • General notes

    A trial size is available to purchase for this antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Predicted molecular weight: 16 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
    • Tissue specificity

      Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
    • Sequence similarities

      Belongs to the IAP family.
      Contains 1 BIR repeat.
    • Developmental stage

      Expression is cell cycle-dependent and peaks at mitosis.
    • Domain

      The BIR repeat is necessary and sufficient for HBXIP binding.
    • Post-translational
      modifications

      Ubiquitination is required for centrosomal targeting.
      In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
    • Cellular localization

      Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • API4 antibody
      • Apoptosis inhibitor 4 antibody
      • Apoptosis inhibitor survivin antibody
      • Apoptosis inhibitor4 antibody
      • Baculoviral IAP repeat containing 5 antibody
      • Baculoviral IAP repeat containing protein 5 antibody
      • Baculoviral IAP repeat-containing protein 5 antibody
      • BIRC 5 antibody
      • BIRC5 antibody
      • BIRC5_HUMAN antibody
      • EPR 1 antibody
      • IAP4 antibody
      • Survivin variant 3 alpha antibody
      • SVV antibody
      • TIAP antibody
      see all

    Images

    • All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/500 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 16 kDa



      Secondary antibody - anti-rabbit HRP (ab6721)

    • All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/300 dilution

      Lane 1 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
      Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 16 kDa


      Exposure time: 1 minute


      Blocking and diluting buffer: 5% NFDM/TBST.

    • Ab134170 staining Survivin in paraffin-embedded rat spleen tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on rat spleen. 

    • Ab134170 staining Survivin in C6 (rat glial tumor glial cell) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in C6 cell line.

    • Ab134170 staining Survivin in paraffin-embedded mouse stomach tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on mouse stomach.

    • Ab134170 staining Survivin in MCF7 (human breast adenocarcinoma epithelial cell line) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in MCF7 cell line. 

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, staining Survivin using ab134170 at a 1/100 dilution.

    References

    This product has been referenced in:

    • Deng S  et al. HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30832202) »
    • Bajbouj K  et al. High-Dose Deferoxamine Treatment Disrupts Intracellular Iron Homeostasis, Reduces Growth, and Induces Apoptosis in Metastatic and Nonmetastatic Breast Cancer Cell Lines. Technol Cancer Res Treat 17:1533033818764470 (2018). Read more (PubMed: 29562821) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (brain with GL261 tumor)
    Antigen retrieval step
    Other
    Permeabilization
    Yes - triton 1%
    Specification
    brain with GL261 tumor
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde

    Mrs. Francoise Geffroy

    Verified customer

    Submitted Aug 02 2019

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 14 2019

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Glioma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer, pH 9
    Permeabilization
    Yes - Triton 0.025%
    Specification
    Glioma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 12 2015

    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (13%)
    Sample
    Mouse Cell lysate - whole cell (MEF cell lysates)
    Specification
    MEF cell lysates
    Treatment
    DMSO control (Ctl) and 7,5 ´M CPT-11 (CPT) for 24 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Christian Marx

    Verified customer

    Submitted Dec 04 2013

    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (13%)
    Sample
    Human Cell lysate - whole cell (HeLa and HCT116 cell lysates)
    Specification
    HeLa and HCT116 cell lysates
    Treatment
    DMSO control (Ctl) and 7,5 ´M CPT-11 (CPT) for 24 hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Christian Marx

    Verified customer

    Submitted Dec 04 2013

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