Recombinant Anti-Survivin antibody [EPR2675] (ab134170)
![Western blot - Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-467563-anti-survivin-antibody-epr2675-westernblot-human-mouse-rat.jpg "Western blot - Anti-Survivin antibody [EPR2675] (ab134170)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2675] to Survivin
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Survivin antibody [EPR2675]
See all Survivin primary antibodies -
Description
Rabbit monoclonal [EPR2675] to Survivin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Survivin aa 50-150. The exact sequence is proprietary.
-
Positive control
- ICC: NIH/3T3 cells; IHC-P: Human tonsil, Mouse and Rat spleen tissue; Flow Cyt: MCF7 cells. WB: HeLa, NIH/3T3, C6, Ramos and F9 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2675 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/200.
|
|
| WB | (2) |
1/300 - 1/1000. Predicted molecular weight: 16 kDa.
|
| IHC-P | (1) |
1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF | (1) |
1/200.
|
| Notes |
|---|
|
Flow Cyt
1/200. |
|
WB
1/300 - 1/1000. Predicted molecular weight: 16 kDa. |
|
IHC-P
1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/200. |
Target
-
Function
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform. -
Tissue specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. -
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat. -
Developmental stage
Expression is cell cycle-dependent and peaks at mitosis. -
Domain
The BIR repeat is necessary and sufficient for HBXIP binding. -
Post-translational
modificationsUbiquitination is required for centrosomal targeting.
In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes. -
Cellular localization
Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
-
Database links
- Entrez Gene: 332 Human
- Entrez Gene: 11799 Mouse
- Entrez Gene: 64041 Rat
- Omim: 603352 Human
- SwissProt: O15392 Human
- SwissProt: O70201 Mouse
- SwissProt: Q9JHY7 Rat
- Unigene: 514527 Human
see all -
Alternative names
- API4 antibody
- Apoptosis inhibitor 4 antibody
- Apoptosis inhibitor survivin antibody
see all
Images
-
Western blot - Anti-Survivin antibody [EPR2675] (ab134170)All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/1000 dilution (Purified)
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100ng/ml nocodazole for 24 hours whole cell lysate
Lane 3 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100ng/ml nocodazole for 24 hours whole cell lysate
Lane 5 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
Lane 6 : C6 (Rat glial tumor glial cell) treated with 100ng/ml nocodazole for 24 hours whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa -
![Western blot - Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-467562-anti-survivin-antibody-epr2675-westernblot-human-mouse.jpg)
Western blot - Anti-Survivin antibody [EPR2675] (ab134170)All lanes : Anti-Survivin antibody [EPR2675] (ab134170) at 1/1000 dilution (Purified)
Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 16 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-13-anti-survivin-antibody-epr2675-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (ab134170)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
![Immunocytochemistry - Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-12-anti-survivin-antibody-epr2675-immunocytochemistry-nih-3t3-mouse.jpg)
Immunocytochemistry - Anti-Survivin antibody [EPR2675] (ab134170)Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling Survivin with purified ab134170 at 1/200 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
![Flow Cytometry - Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-16-anti-survivin-antibody-epr2675-flowcytometry-mcf7-human.jpg)
Flow Cytometry - Anti-Survivin antibody [EPR2675] (ab134170)Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling Survivin with purified ab134170 at 1/200 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-15-anti-survivin-antibody-epr2675-immunohistochemistry-spleen-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (ab134170)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-14-anti-survivin-antibody-epr2675-immunohistochemistry-spleen-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] (ab134170)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
![Anti-Survivin antibody [EPR2675] (ab134170)](https://www.abcam.com/ps/products/134/ab134170/Images/ab134170-10-benefits-of-recombinant-antibodies.png)
Anti-Survivin antibody [EPR2675] (ab134170)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab134170 has been referenced in 10 publications.
- Li C et al. Influence of icariin on inflammation, apoptosis, invasion, and tumor immunity in cervical cancer by reducing the TLR4/MyD88/NF-?B and Wnt/ß-catenin pathways. Cancer Cell Int 21:206 (2021). PubMed: 33849528
- El-Huneidi W et al. Carnosic Acid Induces Apoptosis and Inhibits Akt/mTOR Signaling in Human Gastric Cancer Cell Lines. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 33800129
- El-Huneidi W et al. Micromeria fruticosa Induces Cell Cycle Arrest and Apoptosis in Breast and Colorectal Cancer Cells. Pharmaceuticals (Basel) 13:N/A (2020). PubMed: 32503209
- Huang R et al. The protective effect of polyphyllin I on myocardial ischemia/reperfusion injury in rats. Ann Transl Med 8:644 (2020). PubMed: 32566581
- Li YD et al. RBBP4 promotes colon cancer malignant progression via regulating Wnt/ß-catenin pathway. World J Gastroenterol 26:5328-5342 (2020). PubMed: 32994691
- Deng S et al. HBD Inhibits the Development of Colitis-Associated Cancer in Mice via the IL-6R/STAT3 Signaling Pathway. Int J Mol Sci 20:N/A (2019). PubMed: 30832202
- Bajbouj K et al. Estrogen-dependent disruption of intracellular iron metabolism augments the cytotoxic effects of doxorubicin in select breast and ovarian cancer cells. Cancer Manag Res 11:4655-4668 (2019). PubMed: 31213891
- Talaat IM et al. Potential role for microRNA-16 (miR-16) and microRNA-93 (miR-93) in diagnosis and prediction of disease progression in mycosis fungoides in Egyptian patients. PLoS One 14:e0224305 (2019). PubMed: 31648231
- Bajbouj K et al. High-Dose Deferoxamine Treatment Disrupts Intracellular Iron Homeostasis, Reduces Growth, and Induces Apoptosis in Metastatic and Nonmetastatic Breast Cancer Cell Lines. Technol Cancer Res Treat 17:1533033818764470 (2018). PubMed: 29562821
- Li C et al. SIRT1 expression is associated with poor prognosis of lung adenocarcinoma. Onco Targets Ther 8:977-84 (2015). IHC-P ; Human . PubMed: 25995644