Recombinant Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

Ab134170 staining Survivin in C6 (rat glial tumor glial cell) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor^® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in C6 cell line.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

Ab134170 staining Survivin in MCF7 (human breast adenocarcinoma epithelial cell line) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:500 dilution (2.5µg/ml). An AlexaFluor^®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). Cells were counterstained with an Alexa Fluor^® 594 Anti- Alpha Tubulin [DM1A]- Microtubule Marker at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing strong midbody (arrow) staining in MCF7 cell line. 

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

Ab134170 staining Survivin in paraffin-embedded rat spleen tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on rat spleen. 

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

Ab134170 staining Survivin in paraffin-embedded mouse stomach tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin embedded sections). Samples are incubated in primary antibody at 1:300 dilution (3µg/ml). A Goat Anti-rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1:500 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Hematoxylin was used as a counterstain. Sporadically nuclear staining on mouse stomach.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue, staining Survivin using ab134170 at a 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134170).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.