RecombinantRabMAb

Recombinant Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Immunocytochemistry - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
  • Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2675] to Survivin - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Survivin antibody [EPR2675] - BSA and Azide free
    See all Survivin primary antibodies

  • Description

    Rabbit monoclonal [EPR2675] to Survivin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: NIH/3T3 and HeLa cells; IHC-P: Human tonsil, Mouse and Rat spleen tissue;

  • General notes

    ab167450 is the carrier-free version of ab134170. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab167450 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

    • Tissue specificity

      Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

    • Sequence similarities

      Belongs to the IAP family.
      Contains 1 BIR repeat.

    • Developmental stage

      Expression is cell cycle-dependent and peaks at mitosis.

    • Domain

      The BIR repeat is necessary and sufficient for HBXIP binding.

    • Post-translational
      modifications

      Ubiquitination is required for centrosomal targeting.
      In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.

    • Cellular localization

      Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • API4 antibody
      • Apoptosis inhibitor 4 antibody
      • Apoptosis inhibitor survivin antibody
      • Apoptosis inhibitor4 antibody
      • Baculoviral IAP repeat containing 5 antibody
      • Baculoviral IAP repeat containing protein 5 antibody
      • Baculoviral IAP repeat-containing protein 5 antibody
      • BIRC 5 antibody
      • BIRC5 antibody
      • BIRC5_HUMAN antibody
      • EPR 1 antibody
      • IAP4 antibody
      • Survivin variant 3 alpha antibody
      • SVV antibody
      • TIAP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

      This data was developed using ab134170, the same antibody clone in a different buffer formulation.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunocytochemistry - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
      Immunocytochemistry - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

      This data was developed using ab134170, the same antibody clone in a different buffer formulation.
      Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Bad with purified ab32445 at 1/50 dilution (2.9 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

      This data was developed using ab134170, the same antibody clone in a different buffer formulation.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

      This data was developed using ab134170, the same antibody clone in a different buffer formulation.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling Survivin with purified ab134170 at 1/800 dilution (2.67 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)
      Anti-Survivin antibody [EPR2675] - BSA and Azide free (ab167450)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab167450? Please let us know so that we can cite the reference in this datasheet.

    ab167450 has not yet been referenced specifically in any publications.

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