Overview

  • Product name

  • Description

    Rabbit polyclonal to SUV3L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human SUV3L1 aa 736-786 (C terminal). The exact sequence is proprietary. NP_003162.2
    Sequence:

    RLVQQ GLLTPDMLKQ LEKEWMTQQT EHNKEKTESG THPKGTRRKK KEPDSD


    Database link: Q8IYB8

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab176854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 88 kDa.

Target

  • Function

    Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.
  • Tissue specificity

    Broadly expressed.
  • Sequence similarities

    Belongs to the helicase family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization

    Nucleus. Mitochondrion matrix. Mitochondrion matrix > mitochondrion nucleoid.
  • Information by UniProt
  • Database links

  • Alternative names

    • 6330443E10Rik antibody
    • ATP dependent RNA helicase SUPV3L1, mitochondrial antibody
    • ATP-dependent RNA helicase SUPV3L1 antibody
    • EC 3.6.1. antibody
    • MGC109049 antibody
    • mitochondrial antibody
    • OTTHUMP00000019719 antibody
    • S. Cerevisiae suppressor of var 1 3 like 1 antibody
    • suppressor of var1 (S.cerevisiae) 3 like 1 antibody
    • Suppressor of var1 3 like 1 (S. cerevisiae) antibody
    • Suppressor of var1 3 like 1 (S. cerevisiae)(SUV3) antibody
    • Suppressor of var1 3 like 1 antibody
    • Suppressor of var1 3 like protein 1 antibody
    • Suppressor of var1 3-like protein 1 antibody
    • SUPV 3 L 1 antibody
    • SUPV3L1 antibody
    • SUV 3 antibody
    • SUV 3 L 1 antibody
    • SUV3 antibody
    • SUV3 like protein 1 antibody
    • SUV3-like protein 1 antibody
    • SUV3_HUMAN antibody
    • SUV3L1 antibody
    see all

Images

  • All lanes : Anti-SUV3L1 antibody (ab176854) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 88 kDa


    Exposure time: 30 seconds
  • Detection of SUV3L1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176854 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab176854 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.

References

This product has been referenced in:

  • Toompuu M  et al. Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells. Nucleic Acids Res 46:5209-5226 (2018). Read more (PubMed: 29518244) »
See 1 Publication for this product

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