Key features and details
- Rabbit polyclonal to SUV3L1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SUV3L1 antibody
See all SUV3L1 primary antibodies
DescriptionRabbit polyclonal to SUV3L1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab176854 was affinity purified using an epitope specific to SUV3L1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab176854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 88 kDa.|
FunctionMajor helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.
Tissue specificityBroadly expressed.
Sequence similaritiesBelongs to the helicase family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cellular localizationNucleus. Mitochondrion matrix. Mitochondrion matrix > mitochondrion nucleoid.
- Information by UniProt
- 6330443E10Rik antibody
- ATP dependent RNA helicase SUPV3L1, mitochondrial antibody
- ATP-dependent RNA helicase SUPV3L1 antibody
All lanes : Anti-SUV3L1 antibody (ab176854) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Exposure time: 30 seconds
Detection of SUV3L1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176854 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab176854 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.
ab176854 has been referenced in 1 publication.
- Toompuu M et al. Polyadenylation and degradation of structurally abnormal mitochondrial tRNAs in human cells. Nucleic Acids Res 46:5209-5226 (2018). PubMed: 29518244