Overview

  • Product name
    Anti-Su(var)3-9 antibody
  • Description
    Rabbit polyclonal to Su(var)3-9
  • Host species
    Rabbit
  • Specificity
    ab4811 will recognise KMT1 / Clr4 / Su(var)3-9 but not eIF2-gamma due to immunogen selection.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Fruit fly (Drosophila melanogaster) KMT1/ Clr4/ Su(var)3-9.

    Read Abcam's proprietary immunogen policy (Peptide available as ab87896.)

Properties

Applications

Our Abpromise guarantee covers the use of ab4811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • Relevance
    Histone-lysine N-methyltransferase, H3 lysine-9 specific. Major histone methyltransferase. Methylates Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones. Seems to be involved in heterochromatic gene silencing including the modification of position-effect-variegation. There are three isoforms, isoform A and isoform B share the first 80 amino acid residues. Experimental confirmation may be lacking for some isoforms.
  • Cellular localization
    Nuclear
  • Database links
    • Alternative names
      • H3-K9-HMTase antibody
      • Histone H3-K9 methyltransferase antibody
      • Su(var)3-9 antibody
      • Suppressor of variegation protein 3-9 antibody
      • suv39 antibody
      see all

    References

    This product has been referenced in:
    • Huang XA  et al. A major epigenetic programming mechanism guided by piRNAs. Dev Cell 24:502-16 (2013). Read more (PubMed: 23434410) »
    • Krstic D  et al. Regulated proteolytic processing of Reelin through interplay of tissue plasminogen activator (tPA), ADAMTS-4, ADAMTS-5, and their modulators. PLoS One 7:e47793 (2012). Read more (PubMed: 23082219) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Stylonychia lemnae Cell (single cell organism)
    Specification
    single cell organism
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5%TritonX100/PBS, brief 0.1N HCl treatment, brief pepsin treatment
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jul 20 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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