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  1. Link

    suz12-antibody-ab12073.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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Anti-SUZ12 antibody (ab12073)

  • Datasheet
  • SDS
Reviews (27)Q&A (28)References (134)

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Western blot - Anti-SUZ12 antibody (ab12073)
  • Western blot - Anti-SUZ12 antibody (ab12073)
  • Western blot - Anti-SUZ12 antibody (ab12073)
  • Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)
  • Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)

Key features and details

  • Rabbit polyclonal to SUZ12
  • Suitable for: IP, ChIP, ChIP/Chip, IHC-P, WB, RIP
  • Reacts with: Mouse, Human, Common marmoset
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-SUZ12 antibody [EPR5234(N)] - ChIP Grade (ab175187)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-SUZ12 antibody
    See all SUZ12 primary antibodies
  • Description

    Rabbit polyclonal to SUZ12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, ChIP, ChIP/Chip, IHC-P, WB, RIPmore details
  • Species reactivity

    Reacts with: Mouse, Human, Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Human SUZ12 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab12389)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine methylation

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • KO cell lines

    • Human SUZ12 knockout HeLa cell line (ab264983)
  • KO cell lysates

    • Human SUZ12 knockout HeLa cell lysate (ab257721)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab12073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (3)
Use at an assay dependent concentration.
ChIP (9)
Use at an assay dependent concentration.
ChIP/Chip (1)
Use at an assay dependent concentration.
IHC-P (2)
Use at an assay dependent concentration.
WB (9)
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 83 kDa).
RIP (2)
Use at an assay dependent concentration. PubMed: 19571010
Notes
IP
Use at an assay dependent concentration.
ChIP
Use at an assay dependent concentration.
ChIP/Chip
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 83 kDa).
RIP
Use at an assay dependent concentration. PubMed: 19571010

Target

  • Function

    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Tissue specificity

    Overexpressed in breast and colon cancer.
  • Involvement in disease

    Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
  • Sequence similarities

    Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
    Contains 1 C2H2-type zinc finger.
  • Developmental stage

    Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23512 Human
    • Entrez Gene: 52615 Mouse
    • Omim: 606245 Human
    • SwissProt: Q15022 Human
    • SwissProt: Q80U70 Mouse
    • Unigene: 462732 Human
    • Unigene: 283410 Mouse
    • Unigene: 473315 Mouse
    • Alternative names

      • ChET 9 protein antibody
      • CHET9 antibody
      • Chromatin precipitated E2F target 9 protein antibody
      • JJAZ1 antibody
      • Joined to JAZF1 protein antibody
      • KIAA0160 antibody
      • Polycomb protein SUZ12 antibody
      • Suppressor of zeste 12 homolog antibody
      • Suppressor of zeste 12 protein homolog antibody
      • SUZ12 antibody
      • SUZ12 polycomb repressive complex 2 subunit antibody
      • SUZ12_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SUZ12 antibody (ab12073)
      Western blot - Anti-SUZ12 antibody (ab12073)
      All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml

      Lane 1 : Wild-type Hela cell lysate
      Lane 2 : SUZ12 knockout HeLa cell lysate
      Lane 3 : Wild-type HAP1 cell lysate
      Lane 4 : SUZ12 knockout HAP1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 83 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

      ab12073 was shown to react with SUZ12 in western blot. The band observed in the SUZ12 knockout cell line ab264983 (SUZ12 knockout cell lysate ab257721) lane below 95 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab12073 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-SUZ12 antibody (ab12073)
      Western blot - Anti-SUZ12 antibody (ab12073)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SUZ12 knockout HAP1 cell lysate (20 µg)
      Lane 3: Caco2 cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab12073 was shown to specifically recognize SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab12073 and ab8245 (loading control to GAPDH) were diluted to 1µg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-SUZ12 antibody (ab12073)
      Western blot - Anti-SUZ12 antibody (ab12073)
      All lanes : Anti-SUZ12 antibody (ab12073)

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : SUZ12 CRISPR/Cas9 edited HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 83 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?



      Lanes 1- 2: Merged signal (red and green). Green - ab12073 observed at 95 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab12073 was shown to react with SUZ12 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264983 (CRISPR/Cas9 edited cell lysate ab257721) lane below 95kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and SUZ12 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab12073 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)
      Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)
      All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml

      Lane 1 : untransfected 293T cell lysate
      Lane 2 : 293T cells transfected with 5ug HA-Suz12

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG H&L (HRP) (Dako) at 1/2000 dilution

      Performed under reducing conditions.

      Predicted band size: 83 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?
      Additional bands at: 76 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)
      Western blot - Anti-SUZ12 antibody - ChIP Grade (ab12073)
      All lanes : Anti-SUZ12 antibody (ab12073) at 1 µg/ml

      Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 83 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?
      Additional bands at: 30 kDa, 53 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 4 minutes

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (134)

    Publishing research using ab12073? Please let us know so that we can cite the reference in this datasheet.

    ab12073 has been referenced in 134 publications.

    • Muniz L  et al. Circular ANRIL isoforms switch from repressors to activators of p15/CDKN2B expression during RAF1 oncogene-induced senescence. RNA Biol 18:404-420 (2021). PubMed: 32862732
    • Seo SI  et al. HOTAIR Induces Methylation of PCDH10, a Tumor Suppressor Gene, by Regulating DNMT1 and Sponging with miR-148b in Gastric Adenocarcinoma. Yonsei Med J 62:118-128 (2021). PubMed: 33527791
    • Wei H  et al. OLIG2 regulates lncRNAs and its own expression during oligodendrocyte lineage formation. BMC Biol 19:132 (2021). PubMed: 34172044
    • Zoma M  et al. EZH2-induced lysine K362 methylation enhances TMPRSS2-ERG oncogenic activity in prostate cancer. Nat Commun 12:4147 (2021). PubMed: 34230470
    • Izawa K  et al. PRC2 Components Maintain DNA Hypermethylation of the Upstream Promoter and Regulate Robo4 Expression in Endothelial Cells. Biol Pharm Bull 43:742-746 (2020). PubMed: 32238717
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 55 Abreviews or Q&A

    RIP abreview for Anti-SUZ12 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    RIP
    Sample
    Mouse Cell lysate - whole cell (Th17)
    Negative control
    rabbit IgG
    Immuno-precipitation step
    Protein G
    Specification
    Th17
    Detection step
    Real-time PCR
    Type
    RIP without cross-linking
    Duration of cross-linking step: 0 second(s)
    Positive control
    DDX5
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 24 2022

    ChIP abreview for Anti-SUZ12 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Kidney (Clear Cell Renal Carcinoma))
    Negative control
    Cells with enhancer targeting by CRISPRa.
    Specification
    Kidney (Clear Cell Renal Carcinoma)
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Positive control
    Control cells without enhancer activation (clear cell renal carcinoma 786-O).
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Paulo Rodrigues

    Verified customer

    Submitted Aug 17 2018

    RIP abreview for Anti-SUZ12 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    RIP
    Sample
    Human Tissue lysate - nuclear (BE2C)
    Negative control
    IgG RIP
    Immuno-precipitation step
    Protein A/G
    Specification
    BE2C
    Detection step
    Real-time PCR
    Type
    RIP without cross-linking
    Duration of cross-linking step: 0 second(s)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 12 2017

    Immunoprecipitation abreview for Anti-SUZ12 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein A
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Total protein in input
    20 µg
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 15 2014

    Western blot abreview for Anti-SUZ12 antibody - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (8%)
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Blocking step
    I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 20 2014

    Immunocytochemistry abreview for Anti-SUZ12 antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Blocking step
    3%BSA+0.1% Triton X100 as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
    Sample
    Mouse Cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 18 2014

    ChIP abreview for Anti-SUZ12 antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Detection step
    Real-time PCR
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Negative control
    IgG
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde
    Positive control
    no
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 18 2014

    Immunoprecipitation abreview for Anti-SUZ12 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Immuno-precipitation step
    Other - Magnetic beads
    Sample
    Human Cell lysate - nuclear (RWP1)
    Specification
    RWP1
    Total protein in input
    25 µg
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 10 2014

    Western blot abreview for Anti-SUZ12 antibody - ChIP Grade

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hippocampal primary neurons)
    Gel Running Conditions
    Reduced Denaturing (5%-15%)
    Loading amount
    30 µg
    Specification
    Rat hippocampal primary neurons
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 22 2013

    Western blot abreview for Anti-SUZ12 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (SW-­620)
    Specification
    SW-­620
    Treatment
    tranfect siRNA of SUZ12
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 10 2013

    1-10 of 55 Abreviews or Q&A

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