Overview

  • Product name
    Anti-SUZ12 antibody [EPR5234(N)]
    See all SUZ12 primary antibodies
  • Description
    Rabbit monoclonal [EPR5234(N)] to SUZ12
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SUZ12 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q15022

  • Positive control
    • SW480, HeLa, MCF-7 and 293T cell lysates. MCF-7 cells. Immunoprecipitation pellet from HeLa cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab175187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 83 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/2000.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
    • Tissue specificity
      Overexpressed in breast and colon cancer.
    • Involvement in disease
      Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
    • Sequence similarities
      Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
      Contains 1 C2H2-type zinc finger.
    • Developmental stage
      Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ChET 9 protein antibody
      • CHET9 antibody
      • Chromatin precipitated E2F target 9 protein antibody
      • JJAZ1 antibody
      • Joined to JAZF1 protein antibody
      • KIAA0160 antibody
      • Polycomb protein SUZ12 antibody
      • Suppressor of zeste 12 homolog antibody
      • Suppressor of zeste 12 protein homolog antibody
      • SUZ12 antibody
      • SUZ12 polycomb repressive complex 2 subunit antibody
      • SUZ12_HUMAN antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SUZ12 knockout HAP1 cell lysate (20 µg)
      Lane 3: Caco2 cell lysate (20 µg)
      Lane 4: MCF7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab175187 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.


      ab175187 was shown to specifically react with SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab175187 and ab8245 (loading control to GAPDH) were both 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

    • All lanes : Anti-SUZ12 antibody [EPR5234(N)] (ab175187) at 1/1000 dilution

      Lane 1 : SW480 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : MCF-7 cell lysates
      Lane 4 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 83 kDa

    • Immunofluorescence analysis of MCF-7 cells labeling SUZ12 with ab175187 at a 1/50 dilution.

    • Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SUZ12 (red) with purified ab175187 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175187 at a 1/10 dilution.

    References

    This product has been referenced in:
    • Heubach J  et al. The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells. Mol Cancer 14:108 (2015). WB ; Human . Read more (PubMed: 25994132) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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