Anti-SUZ12 antibody [EPR5234(N)] (ab175187)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-SUZ12 antibody [EPR5234(N)]
See all SUZ12 primary antibodies -
DescriptionRabbit monoclonal [EPR5234(N)] to SUZ12
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human SUZ12 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15022 -
Positive control
- SW480, HeLa, MCF-7 and 293T cell lysates. MCF-7 cells. Immunoprecipitation pellet from HeLa cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR5234(N)
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab175187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 83 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/2000. |
Target
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FunctionPolycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
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Tissue specificityOverexpressed in breast and colon cancer.
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Involvement in diseaseNote=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
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Sequence similaritiesBelongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
Contains 1 C2H2-type zinc finger. -
Developmental stageExpressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 23512 Human
- Entrez Gene: 52615 Mouse
- Entrez Gene: 688041 Rat
- Omim: 606245 Human
- SwissProt: Q15022 Human
- SwissProt: Q80U70 Mouse
- Unigene: 462732 Human
- Unigene: 283410 Mouse
see all -
Alternative names
- ChET 9 protein antibody
- CHET9 antibody
- Chromatin precipitated E2F target 9 protein antibody
see all
Images
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![Western blot - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)](https://www.abcam.com/ps/products/175/ab175187/Images/ab175187-260921-ab175187KOWB.jpg)
Western blot - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SUZ12 knockout HAP1 cell lysate (20 µg)
Lane 3: Caco2 cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab175187 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab175187 was shown to specifically react with SUZ12 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when SUZ12 knockout samples were used. Wild-type and SUZ12 knockout samples were subjected to SDS-PAGE. ab175187 and ab8245 (loading control to GAPDH) were both 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging. -
![Western blot - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)](https://www.abcam.com/ps/products/175/ab175187/Images/ab175187-201482-ab1751871.jpg)
Western blot - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)All lanes : Anti-SUZ12 antibody [EPR5234(N)] (ab175187) at 1/1000 dilution
Lane 1 : SW480 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates
Lane 4 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)](https://www.abcam.com/ps/products/175/ab175187/Images/ab175187-201479-ab1751872.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)Immunofluorescence analysis of MCF-7 cells labeling SUZ12 with ab175187 at a 1/50 dilution.
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![Flow Cytometry - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)](https://www.abcam.com/ps/products/175/ab175187/Images/ab175187-5-ab175187FC1.jpg)
Flow Cytometry - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)Flow cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SUZ12 (red) with purified ab175187 at a 1/2000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunoprecipitation - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)](https://www.abcam.com/ps/products/175/ab175187/Images/ab175187-201477-ab1751873.jpg)
Immunoprecipitation - Anti-SUZ12 antibody [EPR5234(N)] (ab175187)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175187 at a 1/10 dilution.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Heubach J et al. The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells. Mol Cancer 14:108 (2015). WB ; Human . Read more (PubMed: 25994132) »
