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    suz12-antibody-suz220a-ab126577.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation
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Anti-SUZ12 antibody [SUZ220A] (ab126577)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

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Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Flow Cytometry - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)

Key features and details

  • Mouse monoclonal [SUZ220A] to SUZ12
  • Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-SUZ12 antibody [SUZ220A]
    See all SUZ12 primary antibodies
  • Description

    Mouse monoclonal [SUZ220A] to SUZ12
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control

    • IHC: Human tonsil, testicle and thymus tissues; Mouse testicle tissue; V5-tagged Human SUZ12 expressed in Hek293T
      ICC/IF: Human tonsil
      WB: Hek-SUZ12-V5, HeLa, Jeko1, MT-2 and U266 cell lysates
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    SUZ220A
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Polycomb Silencing
    • PRC2
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine methylation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB
1/15. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/30. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB
1/15. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P
1/30. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Tissue specificity

    Overexpressed in breast and colon cancer.
  • Involvement in disease

    Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
  • Sequence similarities

    Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
    Contains 1 C2H2-type zinc finger.
  • Developmental stage

    Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15022 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23512 Human
    • Entrez Gene: 52615 Mouse
    • Omim: 606245 Human
    • SwissProt: Q15022 Human
    • SwissProt: Q80U70 Mouse
    • Unigene: 462732 Human
    • Unigene: 283410 Mouse
    • Unigene: 473315 Mouse
    • Alternative names

      • ChET 9 protein antibody
      • CHET9 antibody
      • Chromatin precipitated E2F target 9 protein antibody
      • JJAZ1 antibody
      • Joined to JAZF1 protein antibody
      • KIAA0160 antibody
      • Polycomb protein SUZ12 antibody
      • Suppressor of zeste 12 homolog antibody
      • Suppressor of zeste 12 protein homolog antibody
      • SUZ12 antibody
      • SUZ12 polycomb repressive complex 2 subunit antibody
      • SUZ12_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577) at 1/100 dilution

      Lane 1 : Hek-SUZ12-V5 lysate at 30 µg
      Lane 2 : Hek-mTIMP2 lysate at 30 µg
      Lane 3 : Hela cell line lysate at 200 µg
      Lane 4 : Jeko1 cell line lysate at 200 µg
      Lane 5 : Human tonsil lysate at 200 µg
      Lane 6 : Human brain lysate at 200 µg
      Lane 7 : MT-2 cell line lysate at 200 µg
      Lane 8 : 3T3 Mouse cell line lysate at 200 µg
      Lane 9 : U266 cell line lysate at 200 µg

      Predicted band size: 83 kDa
      Observed band size: 83 kDa



      Note: A purified version of the antibody was used at 1/100 (stock solution at 1mg/ml).
    • Flow Cytometry - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Flow Cytometry - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Overlay histogram showing MCF7 cells stained with ab126577 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126577, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577)

      Lanes 1 & 4 : Empty vector
      Lanes 2 & 5 : shSUZ12.783
      Lanes 3 & 6 : shSUZ12.2076

      Predicted band size: 83 kDa
      Observed band size: 83 kDa



      Effect of SUZ12 RNA interference (siRNA) in Jeko-1 and Z-138 mantle cell lymphoma cell lines treated with two different hairpins against SUZ12 during three days. A control cell line (empty vector) was used as negative control. Expression of SUZ12 was analyzed using ab126577. A decrease of protein expression was observed in siSUZ12 Jeko-1 and Z-138 cell extracts confirming antibody specificity. Band signals were normalized with tubulin as a loading control.
    • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at 1/500, staining SUZ12 in frozen Human tonsil tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      ab126577, at a 1/30 dilution, staining SUZ12 in paraffin embedded Mouse testicle tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human testicle tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified ab126577 at 1/500 staining SUZ12 in paraffin embedded Human thymus tissue by immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at a 1/100 dilution, staining SUZ12 (green) in Human tonsil by Immunofluorescence. IgD antibody (red) was used at 1/50. After antigen retrieval (Tris-EDTA buffer), the slides were incubated for one hour at room temperature in a humid chamber with primary antibodies. Slides were then washed in PBS 0.5%-Tween20 3 times for 5 minutes each. The slides were incubated for 1 hour with fluorochrome-conjugated antibodies against the different Ig isotypes, diluted in PBS (dilution 1/200) in a humid chamber in the dark. Subsequently, slides were washed in PBS 0.5% Tween20 3 times for 5 minutes each. Following washing, antifading was added.
    • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      To confirm that ab126577 recognized Human SUZ12 protein, Immunohistochemistry on frozen cytospins preparations of V5-tagged Human SUZ12 expressed in Hek293T was performed. Labeling with the ab126577 confirmed the efficiency of transfection. Cytospin preparation of V5-tagged Human BTLA protein was used as a negative control.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab126577? Please let us know so that we can cite the reference in this datasheet.

    ab126577 has been referenced in 5 publications.

    • Wei D  et al. Novel renal medullary carcinoma cell lines, UOK353 and UOK360, provide preclinical tools to identify new therapeutic treatments. Genes Chromosomes Cancer 59:472-483 (2020). PubMed: 32259323
    • Zhu J  et al. Targeting the polycomb repressive complex-2 related proteins with novel combinational strategies for nasopharyngeal carcinoma. Am J Cancer Res 10:3267-3284 (2020). PubMed: 33163269
    • Zhang J  et al. miR-767-5p inhibits glioma proliferation and metastasis by targeting SUZ12. Oncol Rep 42:55-66 (2019). PubMed: 31115583
    • Cho YJ  et al. Prognostic implications of polycomb proteins ezh2, suz12, and eed1 and histone modification by H3K27me3 in sarcoma. BMC Cancer 18:158 (2018). IHC-P ; Human . PubMed: 29415665
    • Zhang M  et al. Somatic mutations of SUZ12 in malignant peripheral nerve sheath tumors. Nat Genet 46:1170-2 (2014). PubMed: 25305755

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    What was the antigen retrieval method used for Immunohistochemistry?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 12 2012

    Answer

    Thank you for contacting us. I apologize for the delay.

    The Suz12 mAb is worked really well in IHC. The buffer that we use is tris-EDTA but we do not use microwave orpresuure cookersince we use a machine (Bondmax, Leica). In any case I am sure that the antibody will work well using both types of antigen retrieval.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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