Overview

  • Product name
    Anti-SUZ12 antibody [SUZ220A]
    See all SUZ12 primary antibodies
  • Description
    Mouse monoclonal [SUZ220A] to SUZ12
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • IHC: Human tonsil, testicle and thymus tissues; Mouse testicle tissue; V5-tagged Human SUZ12 expressed in Hek293T
      ICC/IF: Human tonsil
      WB: Hek-SUZ12-V5, HeLa, Jeko1, MT-2 and U266 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab126577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB 1/15. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/30. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
  • Tissue specificity
    Overexpressed in breast and colon cancer.
  • Involvement in disease
    Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.
  • Sequence similarities
    Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
    Contains 1 C2H2-type zinc finger.
  • Developmental stage
    Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ChET 9 protein antibody
    • CHET9 antibody
    • Chromatin precipitated E2F target 9 protein antibody
    • JJAZ1 antibody
    • Joined to JAZF1 protein antibody
    • KIAA0160 antibody
    • Polycomb protein SUZ12 antibody
    • Suppressor of zeste 12 homolog antibody
    • Suppressor of zeste 12 protein homolog antibody
    • SUZ12 antibody
    • SUZ12 polycomb repressive complex 2 subunit antibody
    • SUZ12_HUMAN antibody
    see all

Images

  • Overlay histogram showing MCF7 cells stained with ab126577 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126577, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577) at 1/100 dilution

    Lane 1 : Hek-SUZ12-V5 lysate at 30 µg
    Lane 2 : Hek-mTIMP2 lysate at 30 µg
    Lane 3 : Hela cell line lysate at 200 µg
    Lane 4 : Jeko1 cell line lysate at 200 µg
    Lane 5 : Human tonsil lysate at 200 µg
    Lane 6 : Human brain lysate at 200 µg
    Lane 7 : MT-2 cell line lysate at 200 µg
    Lane 8 : 3T3 Mouse cell line lysate at 200 µg
    Lane 9 : U266 cell line lysate at 200 µg

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Note: A purified version of the antibody was used at 1/100 (stock solution at 1mg/ml).
  • All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577)

    Lanes 1 & 4 : Empty vector
    Lanes 2 & 5 : shSUZ12.783
    Lanes 3 & 6 : shSUZ12.2076

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Effect of SUZ12 RNA interference (siRNA) in Jeko-1 and Z-138 mantle cell lymphoma cell lines treated with two different hairpins against SUZ12 during three days. A control cell line (empty vector) was used as negative control. Expression of SUZ12 was analyzed using ab126577. A decrease of protein expression was observed in siSUZ12 Jeko-1 and Z-138 cell extracts confirming antibody specificity. Band signals were normalized with tubulin as a loading control.
  • Purified version of ab126577, at 1/500, staining SUZ12 in frozen Human tonsil tissue by Immunohistochemistry.
  • ab126577, at a 1/30 dilution, staining SUZ12 in paraffin embedded Mouse testicle tissue by Immunohistochemistry.
  • Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
  • Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human testicle tissue by Immunohistochemistry.
  • Purified ab126577 at 1/500 staining SUZ12 in paraffin embedded Human thymus tissue by immunohistochemistry.
  • Purified version of ab126577, at a 1/100 dilution, staining SUZ12 (green) in Human tonsil by Immunofluorescence. IgD antibody (red) was used at 1/50. After antigen retrieval (Tris-EDTA buffer), the slides were incubated for one hour at room temperature in a humid chamber with primary antibodies. Slides were then washed in PBS 0.5%-Tween20 3 times for 5 minutes each. The slides were incubated for 1 hour with fluorochrome-conjugated antibodies against the different Ig isotypes, diluted in PBS (dilution 1/200) in a humid chamber in the dark. Subsequently, slides were washed in PBS 0.5% Tween20 3 times for 5 minutes each. Following washing, antifading was added.
  • To confirm that ab126577 recognized Human SUZ12 protein, Immunohistochemistry on frozen cytospins preparations of V5-tagged Human SUZ12 expressed in Hek293T was performed. Labeling with the ab126577 confirmed the efficiency of transfection. Cytospin preparation of V5-tagged Human BTLA protein was used as a negative control.

References

This product has been referenced in:
  • Cho YJ  et al. Prognostic implications of polycomb proteins ezh2, suz12, and eed1 and histone modification by H3K27me3 in sarcoma. BMC Cancer 18:158 (2018). IHC-P ; Human . Read more (PubMed: 29415665) »
  • Zhang M  et al. Somatic mutations of SUZ12 in malignant peripheral nerve sheath tumors. Nat Genet 46:1170-2 (2014). Read more (PubMed: 25305755) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us. I apologize for the delay.

The Suz12 mAb is worked really well in IHC. The buffer that we use is tris-EDTA but we do not use microwave orpresuure cookersince we use a machine (Bondmax, Leica). In any case I am sure that the antibody will work well using both types of antigen retrieval.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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