Anti-SUZ12 antibody [SUZ220A] (ab126577)

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Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Flow Cytometry - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [SUZ220A] (ab126577)
  • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)

Key features and details

  • Mouse monoclonal [SUZ220A] to SUZ12
  • Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-SUZ12 antibody [SUZ220A]
    See all SUZ12 primary antibodies

  • Description

    Mouse monoclonal [SUZ220A] to SUZ12

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein (Human)

  • Positive control

    • IHC: Human tonsil, testicle and thymus tissues; Mouse testicle tissue; V5-tagged Human SUZ12 expressed in Hek293T
      ICC/IF: Human tonsil
      WB: Hek-SUZ12-V5, HeLa, Jeko1, MT-2 and U266 cell lysates

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab126577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
WB 1/15. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/30. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

  • Tissue specificity

    Overexpressed in breast and colon cancer.

  • Involvement in disease

    Note=A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.

  • Sequence similarities

    Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.
    Contains 1 C2H2-type zinc finger.

  • Developmental stage

    Expressed at low levels in quiescent cells. Expression rises at the G1/S phase transition.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • ChET 9 protein antibody
      • CHET9 antibody
      • Chromatin precipitated E2F target 9 protein antibody
      • JJAZ1 antibody
      • Joined to JAZF1 protein antibody
      • KIAA0160 antibody
      • Polycomb protein SUZ12 antibody
      • Suppressor of zeste 12 homolog antibody
      • Suppressor of zeste 12 protein homolog antibody
      • SUZ12 antibody
      • SUZ12 polycomb repressive complex 2 subunit antibody
      • SUZ12_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577) at 1/100 dilution

      Lane 1 : Hek-SUZ12-V5 lysate at 30 µg
      Lane 2 : Hek-mTIMP2 lysate at 30 µg
      Lane 3 : Hela cell line lysate at 200 µg
      Lane 4 : Jeko1 cell line lysate at 200 µg
      Lane 5 : Human tonsil lysate at 200 µg
      Lane 6 : Human brain lysate at 200 µg
      Lane 7 : MT-2 cell line lysate at 200 µg
      Lane 8 : 3T3 Mouse cell line lysate at 200 µg
      Lane 9 : U266 cell line lysate at 200 µg

      Predicted band size: 83 kDa
      Observed band size: 83 kDa



      Note: A purified version of the antibody was used at 1/100 (stock solution at 1mg/ml).
    • Flow Cytometry - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Flow Cytometry - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Overlay histogram showing MCF7 cells stained with ab126577 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126577, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Western blot - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      All lanes : Anti-SUZ12 antibody [SUZ220A] (ab126577)

      Lanes 1 & 4 : Empty vector
      Lanes 2 & 5 : shSUZ12.783
      Lanes 3 & 6 : shSUZ12.2076

      Predicted band size: 83 kDa
      Observed band size: 83 kDa



      Effect of SUZ12 RNA interference (siRNA) in Jeko-1 and Z-138 mantle cell lymphoma cell lines treated with two different hairpins against SUZ12 during three days. A control cell line (empty vector) was used as negative control. Expression of SUZ12 was analyzed using ab126577. A decrease of protein expression was observed in siSUZ12 Jeko-1 and Z-138 cell extracts confirming antibody specificity. Band signals were normalized with tubulin as a loading control.
    • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at 1/500, staining SUZ12 in frozen Human tonsil tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      ab126577, at a 1/30 dilution, staining SUZ12 in paraffin embedded Mouse testicle tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at a 1/500 dilution, staining SUZ12 in paraffin embedded Human testicle tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified ab126577 at 1/500 staining SUZ12 in paraffin embedded Human thymus tissue by immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunocytochemistry/ Immunofluorescence - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Purified version of ab126577, at a 1/100 dilution, staining SUZ12 (green) in Human tonsil by Immunofluorescence. IgD antibody (red) was used at 1/50. After antigen retrieval (Tris-EDTA buffer), the slides were incubated for one hour at room temperature in a humid chamber with primary antibodies. Slides were then washed in PBS 0.5%-Tween20 3 times for 5 minutes each. The slides were incubated for 1 hour with fluorochrome-conjugated antibodies against the different Ig isotypes, diluted in PBS (dilution 1/200) in a humid chamber in the dark. Subsequently, slides were washed in PBS 0.5% Tween20 3 times for 5 minutes each. Following washing, antifading was added.
    • Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      Immunohistochemistry (Frozen sections) - Anti-SUZ12 antibody [SUZ220A] (ab126577)
      To confirm that ab126577 recognized Human SUZ12 protein, Immunohistochemistry on frozen cytospins preparations of V5-tagged Human SUZ12 expressed in Hek293T was performed. Labeling with the ab126577 confirmed the efficiency of transfection. Cytospin preparation of V5-tagged Human BTLA protein was used as a negative control.

    References (3)

    Publishing research using ab126577? Please let us know so that we can cite the reference in this datasheet.

    ab126577 has been referenced in 3 publications.

    • Zhang J  et al. miR-767-5p inhibits glioma proliferation and metastasis by targeting SUZ12. Oncol Rep 42:55-66 (2019). PubMed: 31115583
    • Cho YJ  et al. Prognostic implications of polycomb proteins ezh2, suz12, and eed1 and histone modification by H3K27me3 in sarcoma. BMC Cancer 18:158 (2018). IHC-P ; Human . PubMed: 29415665
    • Zhang M  et al. Somatic mutations of SUZ12 in malignant peripheral nerve sheath tumors. Nat Genet 46:1170-2 (2014). PubMed: 25305755

    Customer reviews and Q&As

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    Question

    What was the antigen retrieval method used for Immunohistochemistry?

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    Answer

    Thank you for contacting us. I apologize for the delay.

    The Suz12 mAb is worked really well in IHC. The buffer that we use is tris-EDTA but we do not use microwave orpresuure cookersince we use a machine (Bondmax, Leica). In any case I am sure that the antibody will work well using both types of antigen retrieval.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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