Key features and details
- Rabbit polyclonal to SV2A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SV2A antibody
See all SV2A primary antibodies
DescriptionRabbit polyclonal to SV2A
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Cynomolgus monkey, Orangutan
- IHC-P: Human cerebral cortex and cerebellum tissue. ICC/IF: U-251 MG cells. WB: Cerebral cortex tissue and U-2 OS cell line.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 83 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
Sequence similaritiesBelongs to the major facilitator superfamily.
modificationsPhosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas.
- Information by UniProt
- KIAA0736 antibody
- OTTHUMP00000014065 antibody
- SV2 antibody
Paraffin-embedded human cerebellum tissue stained for SV2A with ab224073 (1/50 dilution) in immunohistochemical analysis.
Anti-SV2A antibody (ab224073) + Cerebral cortex tissue
Predicted band size: 83 kDa
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SV2A (green) using ab224073 (4 µg/ml) in ICC/IF.
Lane 1 : ab224073 at 0.4 µg/ml
Lane 2 : Anti-SV2A antibody (ab224073) at 0.4 µg/ml
Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cells) cell line
Lane 2 : PC-3 (Human prostate cancer cells) cell line
Predicted band size: 83 kDa
Loading control: Anti-GAPDH
Paraffin-embedded human cerebral cortex tissue stained for SV2A with ab224073 (1/50 dilution) in immunohistochemical analysis.
ab224073 has not yet been referenced specifically in any publications.