Key features and details
- Goat polyclonal to SV2A
- Suitable for: WB, ELISA
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-SV2A antibody
See all SV2A primary antibodies
DescriptionGoat polyclonal to SV2A
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Horse, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey
- Rat brain lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77177 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Our Abpromise guarantee covers the use of ab77177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 83 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPlays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles.
Sequence similaritiesBelongs to the major facilitator superfamily.
modificationsPhosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas.
- Information by UniProt
- KIAA0736 antibody
- OTTHUMP00000014065 antibody
- SV2 antibody
Immunofluorescence analysis of U2OS cells labelling SV2A with ab77177 at 10 µg/mL. Cells were permeabilized with 0.15% Triton X-100. Alexa Fluor 488 secondary antibody (2 µg/mL) was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
All lanes : Anti-SV2A antibody (ab77177) at 0.3 µg/ml
Lane 1 : Rat brain lysate in RIPA buffer
Lane 2 : Rat brain lysate in RIPA buffer treated with immunising peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Primary antibody incubation: 1 hour at room temperature.
An additonal band of unknown identity was also consistently observed at 28kDa, which was successfully blocked by incubation with the immunising peptide.
ab77177 has not yet been referenced specifically in any publications.