Overview

  • Product name

    Anti-SV40 T-antigen antibody [EPR22694-148]
    See all SV40 T-antigen primary antibodies
  • Description

    Rabbit monoclonal [EPR22694-148] to SV40 T-antigen
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details
  • Species reactivity


    Predicted to work with: a wide range of other species, A wide range of other invertebrates
  • Immunogen

    Recombinant fragment within Human SV40 T-antigen aa 250-650. The exact sequence is proprietary.
    Database link: P03070

  • Positive control

    • WB: HEK-293T (transfected) cell lysate. ICC/IF: HEK-293T cells. Flow: HEK-293T (SV40 transfected). IP: HEK-239T whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.
IP 1/30.
Flow Cyt 1/500.
WB 1/1000.

Target

  • Cellular localization

    Nuclear
  • Alternative names

    • Large T antigen antibody
    • LT AG antibody
    • LT antibody
    • LTAG antibody

Images

  • All lanes : Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426) at 1/1000 dilution

    Lane 1 : 293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution


    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Negative control: HEK-293 (PMID: 15175262).

    Exposure time: 10 seconds

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with ab234426 at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in HEK-293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Red) at 1/200 dilution.

    Negative control: HEK-293(PMID: 15175262).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with ab234426 at 1/500 dilution (red), compared with a Rabbit monoclonal IgG isotype control (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.

    Negative control: HEK-293.

  • SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HEK-293T (SV40 transfected) whole cell lysate 10 µg (input).
    Lane 2: ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234426 in HEK-293T whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

References

ab234426 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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