Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22694-148] to SV40 T-antigen
- Suitable for: ICC/IF, IP, Flow Cyt, WB
Product nameAnti-SV40 T-antigen antibody [EPR22694-148]
See all SV40 T-antigen primary antibodies
DescriptionRabbit monoclonal [EPR22694-148] to SV40 T-antigen
Tested applicationsSuitable for: ICC/IF, IP, Flow Cyt, WBmore details
Predicted to work with: a wide range of other species, A wide range of other invertebrates
Recombinant fragment within Human SV40 T-antigen aa 250-650. The exact sequence is proprietary.
Database link: P03070
- WB: HEK-293T (transfected) cell lysate. ICC/IF: HEK-293T cells. Flow: HEK-293T (SV40 transfected). IP: HEK-239T whole cell lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab234426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
All lanes : Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426) at 1/1000 dilution
Lane 1 : 293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Negative control: HEK-293 (PMID: 15175262).
Exposure time: 10 seconds
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with ab234426 at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in HEK-293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Red) at 1/200 dilution.
Negative control: HEK-293(PMID: 15175262).
Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with ab234426 at 1/500 dilution (red), compared with a Rabbit monoclonal IgG isotype control (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.
Negative control: HEK-293.
SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HEK-293T (SV40 transfected) whole cell lysate 10 µg (input).
Lane 2: ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234426 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234426 has not yet been referenced specifically in any publications.