Product nameAnti-SV40 T-antigen antibody [EPR22694-148]
See all SV40 T-antigen primary antibodies
DescriptionRabbit monoclonal [EPR22694-148] to SV40 T-antigen
Tested applicationsSuitable for: ICC/IF, IP, Flow Cyt, WBmore details
Predicted to work with: a wide range of other species, A wide range of other invertebrates
Recombinant fragment within Human SV40 T-antigen aa 250-650. The exact sequence is proprietary.
Database link: P03070
- WB: HEK-293T (transfected) cell lysate. ICC/IF: HEK-293T cells. Flow: HEK-293T (SV40 transfected). IP: HEK-239T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab234426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
All lanes : Anti-SV40 T-antigen antibody [EPR22694-148] (ab234426) at 1/1000 dilution
Lane 1 : 293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Negative control: HEK-293 (PMID: 15175262).
Exposure time: 10 seconds
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labeling SV40 T-antigen with ab234426 at 1/50 dilution, followed by an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) (Green) at 1/1000 dilution. Confocal image showing nuclear and weak cytoplasmic staining in HEK-293T cells. The nuclear counterstain is DAPI (Blue). Tubulin was stained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Red) at 1/200 dilution.
Negative control: HEK-293(PMID: 15175262).
Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293 (human embryonic kidney epithelial cell, Left) / 293T (SV40 transfected human embryonic kidney epithelial cell, Right) cells labeling SV40 T-antigen with ab234426 at 1/500 dilution (red), compared with a Rabbit monoclonal IgG isotype control (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/2000 dilution.
Negative control: HEK-293.
SV40 T-antigen was immunoprecipitated from 0.35 mg HEK-293T (SV40 transfected human embryonic kidney epithelial cell) whole cell lysate with ab234426 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab234426 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HEK-293T (SV40 transfected) whole cell lysate 10 µg (input).
Lane 2: ab234426 IP in HEK-293T (SV40 transfected) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234426 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
ab234426 has not yet been referenced specifically in any publications.