Anti-SV40 T-antigen antibody [PAb416] (ab16879)

Product name

Anti-SV40 T-antigen antibody [PAb416]
See all SV40 T-antigen primary antibodies
Description

Mouse monoclonal [PAb416] to SV40 T-antigen
Host species

Mouse
Tested applications

Suitable for: ICC, Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
Species reactivity

SV40 infected cells.
Immunogen

SV40 transformed mouse cells
Epitope

Within amino acids 83-128 of the large T-antigen.
Positive control
- SV80 or SV-T2 cells.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Preservative: 0.02% Sodium azide
Constituent: PBS
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

PAb416
Myeloma

NS1
Isotype

IgG2a
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit (ab102918)
- APC Conjugation Kit (ab201807)

Our Abpromise guarantee covers the use of ab16879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC		Use a concentration of 1 - 5 µg/ml.
Flow Cyt		1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF		Use a concentration of 1 - 5 µg/ml.
WB		Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
IP		Use a concentration of 1 - 5 µg/ml.
IHC-P		Use at an assay dependent concentration.

Cellular localization

Nuclear
Alternative names
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody

Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an Abreview submitted by Mr Adam Hoffhines

All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml

Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.

Lysates/proteins at 7 µg per lane.

Secondary
All lanes : HRP conjugated sheep-anti mouse IgG

Developed using the ECL technique.

Performed under non-reducing conditions.

Predicted band size: 82 kDa
Observed band size: ~80 kDa
why is the actual band size different from the predicted?

Exposure time: 2 seconds
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an anonymous Abreview

ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.
See Abreview
Flow Cytometry - Anti-SV40 T-antigen antibody [PAb416] (ab16879)Image courtesy of Nir Drayman by Abreview.

CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were then harvested (trypsinized and centrifuged) and fixed 72 hours post infection in 100% Methanol at -20°C. Cells were stained for 1 hour with ab16879 at a 1/100 dilution, washed and then stained with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed, ab98794 at a 1/200 dilution.
See Abreview

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Edwards TG & Fisher C Antiviral activity of pyrrole-imidazole polyamides against SV40 and BK polyomaviruses. Antiviral Res 152:68-75 (2018). ICC/IF . Read more (PubMed: 29458134) »
Panou MM et al. Agnoprotein Is an Essential Egress Factor during BK Polyomavirus Infection. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29562663) »

See all 17 Publications for this product

Publishing research using ab16879? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (hCMEC-D3)

Permeabilization

Yes - TRITON 1%

Specification

hCMEC-D3

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

Mrs. Francoise Geffroy

Verified customer

Submitted May 11 2017

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Flow Cytometry

Sample

African Green Monkey Cell (Simian virus 40 (SV40))

Specification

Simian virus 40 (SV40)

Fixation

Methanol

Permeabilization

Yes - Methanol

Mr. Nir Drayman

Verified customer

Submitted Aug 10 2011

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Human Cell lysate - whole cell (Human Renal Proximal Tubular cells)

Loading amount

10 µg

Specification

Human Renal Proximal Tubular cells

Gel Running Conditions

Reduced Denaturing (10 %)

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. Robert Jenkins

Verified customer

Submitted Jun 03 2009

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Mouse Cell lysate - whole cell (fibroblast tumor cell)

Loading amount

10 µg

Specification

fibroblast tumor cell

Gel Running Conditions

Reduced Denaturing (4-15%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Mr. Manish Garg

Verified customer

Submitted Oct 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Mouse Cell lysate - whole cell (SPLEEN)

Loading amount

30 µg

Specification

SPLEEN

Gel Running Conditions

Reduced Denaturing (4-12%)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts (MEFs))

Loading amount

7 µg

Specification

Mouse Embryonic Fibroblasts (MEFs)

Gel Running Conditions

Non-reduced Denaturing (4-12% Bis-Tris gradient gel)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Adam Hoffhines

Verified customer

Submitted Mar 25 2008

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (transfected keratinocyte)

Specification

transfected keratinocyte

Fixative

Paraformaldehyde

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Sep 08 2006

Question

When this antibody was tested in WB, what was the specific protocol that was used?

Abcam community

Verified customer

Asked on Nov 14 2005

Answer

Thank you for your enquiry. This clone was originally characterized in the following publication, Harlow, E., et al. (1981. J. Virol. 39:861, free on-line), the authors originally used this antibody (referred to as L16 in the original publication) to immunoprecipitate 32P-pulsed SV80 cells, then detect the IP by SDS-PAGE, followed by autoradiography. This clone has been characterized for application in Western blotting; however, the exact protocol that was used is not available. We do guarantee that ab16879 will work in Western blotting, and often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=16879&mode=questionaire

Abcam Scientific Support

Answered on Nov 16 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-SV40 T-antigen antibody [PAb416] (ab16879)

Overview

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References

This product has been referenced in:

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-SV40 T-antigen antibody [PAb416]

Flow Cytometry abreview for Anti-SV40 T-antigen antibody [PAb416]

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Immunocytochemistry/ Immunofluorescence abreview for Anti-SV40 T-antigen antibody [PAb416]