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  1. Link

    sv40-t-antigen-antibody-pab416-ab16879.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Other dsDNA viruses
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Anti-SV40 T-antigen antibody [PAb416] (ab16879)

  • Datasheet
  • SDS
Reviews (7)Q&A (1)References (32)

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Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)
  • Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)

Key features and details

  • Mouse monoclonal [PAb416] to SV40 T-antigen
  • Suitable for: ICC/IF, WB
  • Reacts with: Species independent
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-SV40 T-antigen antibody [PAb416]
    See all SV40 T-antigen primary antibodies
  • Description

    Mouse monoclonal [PAb416] to SV40 T-antigen
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    SV40 transformed mouse cells

  • Epitope

    Within amino acids 83-128 of the large T-antigen.
  • Positive control

    • SV80 or SV-T2 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    PAb416
  • Myeloma

    NS1
  • Isotype

    IgG2a
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Other dsDNA viruses
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • SV40 antigen

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
Use a concentration of 1 - 5 µg/ml.
WB (4)
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.

Target

  • Cellular localization

    Nuclear
  • Alternative names

    • Large T antigen antibody
    • LT AG antibody
    • LT antibody
    • LTAG antibody

Images

  • Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)
    Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an Abreview submitted by Mr Adam Hoffhines
    All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml

    Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
    Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.

    Lysates/proteins at 7 µg per lane.

    Secondary
    All lanes : HRP conjugated sheep-anti mouse IgG

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: ~80 kDa why is the actual band size different from the predicted?


    Exposure time: 2 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)
    Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an anonymous Abreview
     ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.

    See Abreview

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (32)

Publishing research using ab16879? Please let us know so that we can cite the reference in this datasheet.

ab16879 has been referenced in 32 publications.

  • Ferreira CB  et al. Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity. Mol Ther Methods Clin Dev 17:209-219 (2020). PubMed: 31970199
  • Graubner FR  et al. Canine decidualization in vitro: extracellular matrix modification, progesterone mediated effects and selective blocking of prostaglandin E2 receptors. J Reprod Dev 66:319-329 (2020). PubMed: 32201411
  • Low JY  et al. Stromal CAVIN1 Controls Prostate Cancer Microenvironment and Metastasis by Modulating Lipid Distribution and Inflammatory Signaling. Mol Cancer Res 18:1414-1426 (2020). PubMed: 32493699
  • Qi X  et al. An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis. J Vis Exp N/A:N/A (2019). PubMed: 31566616
  • Tarnita RM  et al. Contribution of DNA Replication to the FAM111A-Mediated Simian Virus 40 Host Range Phenotype. J Virol 93:N/A (2019). PubMed: 30333173
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SV40 T-antigen antibody [PAb416]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hCMEC-D3)
Permeabilization
Yes - TRITON 1%
Specification
hCMEC-D3
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Mrs. Francoise Geffroy

Verified customer

Submitted May 11 2017

Flow Cytometry abreview for Anti-SV40 T-antigen antibody [PAb416]

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
African Green Monkey Cell (Simian virus 40 (SV40))
Specification
Simian virus 40 (SV40)
Fixation
Methanol
Permeabilization
Yes - Methanol
Read More

Mr. Nir Drayman

Verified customer

Submitted Aug 10 2011

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Renal Proximal Tubular cells)
Loading amount
10 µg
Specification
Human Renal Proximal Tubular cells
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Dr. Robert Jenkins

Verified customer

Submitted Jun 03 2009

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast tumor cell)
Loading amount
10 µg
Specification
fibroblast tumor cell
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

Mr. Manish Garg

Verified customer

Submitted Oct 01 2008

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (SPLEEN)
Loading amount
30 µg
Specification
SPLEEN
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted May 22 2008

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts (MEFs))
Loading amount
7 µg
Specification
Mouse Embryonic Fibroblasts (MEFs)
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Mr. Adam Hoffhines

Verified customer

Submitted Mar 25 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-SV40 T-antigen antibody [PAb416]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (transfected keratinocyte)
Specification
transfected keratinocyte
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

Submitted Sep 08 2006

Question

When this antibody was tested in WB, what was the specific protocol that was used?

Read More

Abcam community

Verified customer

Asked on Nov 14 2005

Answer

Thank you for your enquiry. This clone was originally characterized in the following publication, Harlow, E., et al. (1981. J. Virol. 39:861, free on-line), the authors originally used this antibody (referred to as L16 in the original publication) to immunoprecipitate 32P-pulsed SV80 cells, then detect the IP by SDS-PAGE, followed by autoradiography. This clone has been characterized for application in Western blotting; however, the exact protocol that was used is not available. We do guarantee that ab16879 will work in Western blotting, and often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=16879&mode=questionaire

Read More

Abcam Scientific Support

Answered on Nov 16 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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