Anti-SV40 T-antigen antibody [PAb416] (ab16879)
Key features and details
- Mouse monoclonal [PAb416] to SV40 T-antigen
- Suitable for: ICC, ICC/IF, WB, IP, IHC-P
- Isotype: IgG2a
Overview
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Product name
Anti-SV40 T-antigen antibody [PAb416]
See all SV40 T-antigen primary antibodies -
Description
Mouse monoclonal [PAb416] to SV40 T-antigen -
Host species
Mouse -
Tested applications
Suitable for: ICC, ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
SV40 infected cells. -
Immunogen
SV40 transformed mouse cells
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Epitope
Within amino acids 83-128 of the large T-antigen. -
Positive control
- SV80 or SV-T2 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
PAb416 -
Myeloma
NS1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab16879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use a concentration of 1 - 5 µg/ml. | |
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa. | |
| IP | Use a concentration of 1 - 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Cellular localization
Nuclear -
Alternative names
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
Images
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![Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.com/ps/products/16/ab16879/Images/ab16879-25763-anti-sv40-t-antigen-antibody-pab416-western-blot.jpg)
Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an Abreview submitted by Mr Adam HoffhinesAll lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
All lanes : HRP conjugated sheep-anti mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 82 kDa
Observed band size: ~80 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.com/ps/products/16/ab16879/Images/ab16879-25764-anti-sv40-t-antigen-antibody-pab416-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an anonymous Abreviewab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.
Protocols
References (27)
ab16879 has been referenced in 27 publications.
- Ferreira CB et al. Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity. Mol Ther Methods Clin Dev 17:209-219 (2020). PubMed: 31970199
- Gram A et al. Lipopolysaccharide disrupts gap junctional intercellular communication in an immortalized ovine luteal endothelial cell line. Toxicol In Vitro 60:437-449 (2019). PubMed: 31154062
- Kajon AE et al. Isolation and initial propagation of guinea pig adenovirus (GPAdV) in Cavia porcellus cell lines. F1000Res 8:1597 (2019). PubMed: 32226606
- Tarnita RM et al. Contribution of DNA Replication to the FAM111A-Mediated Simian Virus 40 Host Range Phenotype. J Virol 93:N/A (2019). PubMed: 30333173
- Caller LG et al. Temporal Proteomic Analysis of BK Polyomavirus Infection Reveals Virus-Induced G2 Arrest and Highly Effective Evasion of Innate Immune Sensing. J Virol 93:N/A (2019). PubMed: 31142673
