Anti-SV40 T-antigen antibody [PAb416] (ab16879)

Mouse monoclonal SV40 T-antigen antibody [PAb416]. Validated in WB, IP, IHC, ICC, Flow Cyt, ICC/IF. Cited in 17 publication(s). Independently reviewed in 7 review(s).

Reviews (7)Q&A (1)References (22)

Overview

  • Product name

    Anti-SV40 T-antigen antibody [PAb416]
    See all SV40 T-antigen primary antibodies

  • Description

    Mouse monoclonal [PAb416] to SV40 T-antigen

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details

  • Species reactivity

    SV40 infected cells.

  • Immunogen

    SV40 transformed mouse cells

  • Epitope

    Within amino acids 83-128 of the large T-antigen.

  • Positive control

    • SV80 or SV-T2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab16879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 - 5 µg/ml.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
IP Use a concentration of 1 - 5 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Cellular localization

    Nuclear

  • Alternative names

    • Large T antigen antibody
    • LT AG antibody
    • LT antibody
    • LTAG antibody

Images

  • Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)
    Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an Abreview submitted by Mr Adam Hoffhines
    All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml

    Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
    Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.

    Lysates/proteins at 7 µg per lane.

    Secondary
    All lanes : HRP conjugated sheep-anti mouse IgG

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 82 kDa
    Observed band size: ~80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)
    Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an anonymous Abreview
     ab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.

    See Abreview

  • Flow Cytometry - Anti-SV40 T-antigen antibody [PAb416] (ab16879)
    Flow Cytometry - Anti-SV40 T-antigen antibody [PAb416] (ab16879)Image courtesy of Nir Drayman by Abreview.
    CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were then harvested (trypsinized and centrifuged) and fixed 72 hours post infection in 100% Methanol at -20°C. Cells were stained for 1 hour with ab16879 at a 1/100 dilution, washed and then stained with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed, ab98794 at a 1/200 dilution.

    See Abreview

References

This product has been referenced in:

  • Burkard M  et al. Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor). Cell Biol Toxicol N/A:N/A (2019). Read more (PubMed: 30627956) »
  • Kittipassorn T  et al. Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1. Exp Eye Res 181:127-135 (2019). Read more (PubMed: 30677389) »
See all 22 Publications for this product

Publishing research using ab16879? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SV40 T-antigen antibody [PAb416]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hCMEC-D3)
Permeabilization
Yes - TRITON 1%
Specification
hCMEC-D3
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Mrs. Francoise Geffroy

Verified customer

Submitted May 11 2017

Flow Cytometry abreview for Anti-SV40 T-antigen antibody [PAb416]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
African Green Monkey Cell (Simian virus 40 (SV40))
Specification
Simian virus 40 (SV40)
Fixation
Methanol
Permeabilization
Yes - Methanol
Read More

Mr. Nir Drayman

Verified customer

Submitted Aug 10 2011

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Renal Proximal Tubular cells)
Loading amount
10 µg
Specification
Human Renal Proximal Tubular cells
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Dr. Robert Jenkins

Verified customer

Submitted Jun 03 2009

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast tumor cell)
Loading amount
10 µg
Specification
fibroblast tumor cell
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More

Mr. Manish Garg

Verified customer

Submitted Oct 01 2008

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (SPLEEN)
Loading amount
30 µg
Specification
SPLEEN
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Abcam user community

Verified customer

Submitted May 22 2008

Western blot abreview for Anti-SV40 T-antigen antibody [PAb416]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts (MEFs))
Loading amount
7 µg
Specification
Mouse Embryonic Fibroblasts (MEFs)
Gel Running Conditions
Non-reduced Denaturing (4-12% Bis-Tris gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More

Mr. Adam Hoffhines

Verified customer

Submitted Mar 25 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-SV40 T-antigen antibody [PAb416]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (transfected keratinocyte)
Specification
transfected keratinocyte
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More

Abcam user community

Verified customer

Submitted Sep 08 2006

Question

When this antibody was tested in WB, what was the specific protocol that was used?

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Answer

Thank you for your enquiry. This clone was originally characterized in the following publication, Harlow, E., et al. (1981. J. Virol. 39:861, free on-line), the authors originally used this antibody (referred to as L16 in the original publication) to immunoprecipitate 32P-pulsed SV80 cells, then detect the IP by SDS-PAGE, followed by autoradiography. This clone has been characterized for application in Western blotting; however, the exact protocol that was used is not available. We do guarantee that ab16879 will work in Western blotting, and often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=16879&mode=questionaire

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