Anti-SV40 T-antigen antibody [PAb416] (ab16879)
![Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.com/ps/products/16/ab16879/Images/ab16879-25763-anti-sv40-t-antigen-antibody-pab416-western-blot.jpg "Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)")
Key features and details
- Mouse monoclonal [PAb416] to SV40 T-antigen
- Suitable for: ICC, ICC/IF, WB, IP, IHC-P
- Isotype: IgG2a
Overview
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Product name
Anti-SV40 T-antigen antibody [PAb416]
See all SV40 T-antigen primary antibodies -
Description
Mouse monoclonal [PAb416] to SV40 T-antigen -
Host species
Mouse -
Tested applications
Suitable for: ICC, ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
SV40 infected cells. -
Immunogen
SV40 transformed mouse cells
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Epitope
Within amino acids 83-128 of the large T-antigen. -
Positive control
- SV80 or SV-T2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
PAb416 -
Myeloma
NS1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 1 - 5 µg/ml.
|
|
| ICC/IF | (2) |
Use a concentration of 1 - 5 µg/ml.
|
| WB | (4) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
|
| IP |
Use a concentration of 1 - 5 µg/ml.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC
Use a concentration of 1 - 5 µg/ml. |
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa. |
|
IP
Use a concentration of 1 - 5 µg/ml. |
|
IHC-P
Use at an assay dependent concentration. |
Target
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Cellular localization
Nuclear -
Alternative names
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
Images
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Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an Abreview submitted by Mr Adam HoffhinesAll lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
All lanes : HRP conjugated sheep-anti mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 82 kDa
Observed band size: ~80 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.com/ps/products/16/ab16879/Images/ab16879-25764-anti-sv40-t-antigen-antibody-pab416-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an anonymous Abreviewab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab16879 has been referenced in 28 publications.
- Ferreira CB et al. Lentiviral Vector Production Titer Is Not Limited in HEK293T by Induced Intracellular Innate Immunity. Mol Ther Methods Clin Dev 17:209-219 (2020). PubMed: 31970199
- Gram A et al. Lipopolysaccharide disrupts gap junctional intercellular communication in an immortalized ovine luteal endothelial cell line. Toxicol In Vitro 60:437-449 (2019). PubMed: 31154062
- Kajon AE et al. Isolation and initial propagation of guinea pig adenovirus (GPAdV) in Cavia porcellus cell lines. F1000Res 8:1597 (2019). PubMed: 32226606
- Oka M et al. Hepatocyte Growth Factor-Secreting Mesothelial Cell Sheets Suppress Progressive Fibrosis in a Rat Model of CKD. J Am Soc Nephrol 30:261-276 (2019). PubMed: 30635373
- Tarnita RM et al. Contribution of DNA Replication to the FAM111A-Mediated Simian Virus 40 Host Range Phenotype. J Virol 93:N/A (2019). PubMed: 30333173