Anti-SV40 T-antigen antibody [PAb416] (ab16879)
Mouse monoclonal SV40 T-antigen antibody [PAb416]. Validated in WB, IP, IHC, ICC, Flow Cyt, ICC/IF. Cited in 17 publication(s). Independently reviewed in 7 review(s).
- Datasheet
- References (22)
- Protocols
Overview
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Product name
Anti-SV40 T-antigen antibody [PAb416]
See all SV40 T-antigen primary antibodies -
Description
Mouse monoclonal [PAb416] to SV40 T-antigen -
Host species
Mouse -
Tested applications
Suitable for: ICC, Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
SV40 infected cells. -
Immunogen
SV40 transformed mouse cells
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Epitope
Within amino acids 83-128 of the large T-antigen. -
Positive control
- SV80 or SV-T2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
PAb416 -
Myeloma
NS1 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab16879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use a concentration of 1 - 5 µg/ml. | |
| Flow Cyt | 1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
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| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa. | |
| IP | Use a concentration of 1 - 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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Cellular localization
Nuclear -
Alternative names
- Large T antigen antibody
- LT AG antibody
- LT antibody
- LTAG antibody
Images
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![Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.com/ps/products/16/ab16879/Images/ab16879-25763-anti-sv40-t-antigen-antibody-pab416-western-blot.jpg)
Western blot - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an Abreview submitted by Mr Adam HoffhinesAll lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml
Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen.
Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.
Lysates/proteins at 7 µg per lane.
Secondary
All lanes : HRP conjugated sheep-anti mouse IgG
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 82 kDa
Observed band size: ~80 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.com/ps/products/16/ab16879/Images/ab16879-25764-anti-sv40-t-antigen-antibody-pab416-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SV40 T-antigen antibody [PAb416] (ab16879)This image is courtesy of an anonymous Abreviewab16879 at a 1/50 dilution staining SV40 transfected mouse keratinocytes by ICC/IF. -
![Flow Cytometry - Anti-SV40 T-antigen antibody [PAb416] (ab16879)](https://www.abcam.com/ps/products/16/ab16879/Images/ab16879-25762-anti-sv40-t-antigen-antibody-pab416-flow-cytometry.jpg)
Flow Cytometry - Anti-SV40 T-antigen antibody [PAb416] (ab16879)Image courtesy of Nir Drayman by Abreview.CV1 cells were either mock infected or infected with SV40 at moi 10. Cells were then harvested (trypsinized and centrifuged) and fixed 72 hours post infection in 100% Methanol at -20°C. Cells were stained for 1 hour with ab16879 at a 1/100 dilution, washed and then stained with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed, ab98794 at a 1/200 dilution.
Protocols
References
This product has been referenced in:
- Burkard M et al. Lifetime extension of humpback whale skin fibroblasts and their response to lipopolysaccharide (LPS) and a mixture of polychlorinated biphenyls (Aroclor). Cell Biol Toxicol N/A:N/A (2019). Read more (PubMed: 30627956) »
- Kittipassorn T et al. Characterization of the novel spontaneously immortalized rat Müller cell line SIRMu-1. Exp Eye Res 181:127-135 (2019). Read more (PubMed: 30677389) »
