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When this antibody was tested in WB, what was the specific protocol that was used?
Asked on Nov 14 2005
Thank you for your enquiry. This clone was originally characterized in the following publication, Harlow, E., et al. (1981. J. Virol. 39:861, free on-line), the authors originally used this antibody (referred to as L16 in the original publication) to immunoprecipitate 32P-pulsed SV80 cells, then detect the IP by SDS-PAGE, followed by autoradiography. This clone has been characterized for application in Western blotting; however, the exact protocol that was used is not available. We do guarantee that ab16879 will work in Western blotting, and often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to a questionnaire which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=16879&mode=questionaire
Answered on Nov 16 2005