Product nameAnti-SV40 VP1 antibody
DescriptionRabbit polyclonal to SV40 VP1
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Simian Virus 40
Recombinant fusion protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab53977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Predicted molecular weight: 40 kDa.|
|IP||Use at an assay dependent dilution.|
|ICC/IF||1/1000 - 1/5000.|
RelevanceVP1 is one of three simian virus 40 (SV40) capsid proteins. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid.
- Capsid protein VP1 antibody
All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution
Lane 1 : BK virus infected 293TT whole cell lysate
Lane 2 : Mock infected 293TT whole cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.
ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.
This product has been referenced in:
- Tsai K et al. Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication. PLoS Pathog 14:e1006919 (2018). Read more (PubMed: 29447282) »
- Toscano MG et al. Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies. Mol Ther Methods Clin Dev 6:124-134 (2017). WB . Read more (PubMed: 28791314) »