• Product name

    Anti-SV40 VP1 antibody
  • Description

    Rabbit polyclonal to SV40 VP1
  • Host species

  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Simian Virus 40
  • Immunogen

    Recombinant fusion protein



Our Abpromise guarantee covers the use of ab53977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.


  • Relevance

    VP1 is one of three simian virus 40 (SV40) capsid proteins. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid.
  • Alternative names

    • Capsid protein VP1 antibody


  • All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution

    Lane 1 : BK virus infected 293TT whole cell lysate
    Lane 2 : Mock infected 293TT whole cell lysate

    Lysates/proteins at 15 µg per lane.

    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?

    Exposure time: 4 minutes

    See Abreview

  • SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
  • ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.
  • ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.


This product has been referenced in:

  • Tsai K  et al. Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication. PLoS Pathog 14:e1006919 (2018). Read more (PubMed: 29447282) »
  • Toscano MG  et al. Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies. Mol Ther Methods Clin Dev 6:124-134 (2017). WB . Read more (PubMed: 28791314) »
See all 12 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Simian Virus 40 Serum (Vector particles produced from packaging cells. Vi)
Vector particles produced from packaging cells. Vi
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 25°C

Miguel Garcia

Verified customer

Submitted Jan 24 2017

Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (10%)
Human Cell lysate - whole cell (BK virus infected 293TT cells)
BK virus infected 293TT cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 18 2014


Thank you for your enquiry and for your patience. We aim to get back to customers within 24 hours and I am sorry this did not happen on this occasion.

I have attached the MSDS for ab53977. This is now also available on the datasheet.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

Read More



The supplier of the antibody was unable to provide a concentration, although I can tell you for rabbit antibodies typically the IgG is 12 - 15 mg/ml. If you did need a more specific concentration you could purify the antibody with a kit such as our Antibody Serum Purification Kit ab109209 (which uses Protein A) and then test the concentration yourself. Please let me know if I can be of further assistance.

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Thank you for your enquiry. I can confirm that ab53977 SV40 VP1 antibody detects infecting SV40 in the cell during the first hours of infection, before new VP1 is expressed from the incoming virus. I am sorry it has not yet been tested tested it against BK infection. We would assume it would behave the same since it cross reacts with BK and since their capsid structure is similar. However, we can't guarantee it without further testing. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Thank you for your enquiry. I have received confirmation from the originator of the product that this ab53977 do cross-react with JC and BK, and in-fact give very good signals with pathological specimens. However, it cannot be used to distinguish between these viruses. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me.

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