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  1. Link

    sv40-vp1-antibody-ab53977.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Other dsDNA viruses
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Anti-SV40 VP1 antibody (ab53977)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (12)

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  • Western blot - Anti-SV40 VP1 antibody (ab53977)
  • Western blot - Anti-SV40 VP1 antibody (ab53977)
  • Immunoprecipitation - Anti-SV40 VP1 antibody (ab53977)
  • Immunocytochemistry/ Immunofluorescence - Anti-SV40 VP1 antibody (ab53977)

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  • Datasheet
  • References (12)
  • Protocols

Overview

  • Product name

    Anti-SV40 VP1 antibody
  • Description

    Rabbit polyclonal to SV40 VP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Simian Virus 40
  • Immunogen

    Recombinant fusion protein

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Other dsDNA viruses
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • SV40 antigen

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab53977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.

Target

  • Relevance

    VP1 is one of three simian virus 40 (SV40) capsid proteins. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid.
  • Alternative names

    • Capsid protein VP1 antibody

Images

  • Western blot - Anti-SV40 VP1 antibody (ab53977)
    Western blot - Anti-SV40 VP1 antibody (ab53977)
    All lanes : Anti-SV40 VP1 antibody (ab53977) at 1/5000 dilution

    Lane 1 : BK virus infected 293TT whole cell lysate
    Lane 2 : Mock infected 293TT whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes

    See Abreview

  • Western blot - Anti-SV40 VP1 antibody (ab53977)
    Western blot - Anti-SV40 VP1 antibody (ab53977)
    SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
  • Immunoprecipitation - Anti-SV40 VP1 antibody (ab53977)
    Immunoprecipitation - Anti-SV40 VP1 antibody (ab53977)
    ab53977 detecting VP1 by Immunoprecipitation. Mock or SV40-transfected CV1 cells were lysed with RIPA buffer. 100 µg total protein from each lysate was pre-cleared for 1 hour and then immunoprecipitated with alpha-VP1 1/1000 overnight at 4°C. Input and IP samples were run on an SDS gel and detected with alpha-VP1 diluted 1/10,000 using the ECL method.
  • Immunocytochemistry/ Immunofluorescence - Anti-SV40 VP1 antibody (ab53977)
    Immunocytochemistry/ Immunofluorescence - Anti-SV40 VP1 antibody (ab53977)
    ab53977 at 1/1000 dilution, staining SV40 VP1 in transfected cells. Cells were transfected with SV40 or mock-transfected, fixed for 5 minutes after adsorption in 100% methanol, blocked and stained with the antibody for 1 hour at room temperature. Cells were washed and stained with an Alexa Fluor® 488-conjugated anti-rabbit antibody. DAPI was used to counterstain the nuclei.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References

    This product has been referenced in:

    • Tsai K  et al. Addition of m6A to SV40 late mRNAs enhances viral structural gene expression and replication. PLoS Pathog 14:e1006919 (2018). Read more (PubMed: 29447282) »
    • Toscano MG  et al. Generation of a Vero-Based Packaging Cell Line to Produce SV40 Gene Delivery Vectors for Use in Clinical Gene Therapy Studies. Mol Ther Methods Clin Dev 6:124-134 (2017). WB . Read more (PubMed: 28791314) »
    See all 12 Publications for this product

    Publishing research using ab53977? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews

    ELISA abreview for Anti-SV40 VP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Simian Virus 40 Serum (Vector particles produced from packaging cells. Vi)
    Specification
    Vector particles produced from packaging cells. Vi
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Direct
    Read More

    Miguel Garcia

    Verified customer

    Submitted Jan 24 2017

    Western blot abreview for Anti-SV40 VP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Human Cell lysate - whole cell (BK virus infected 293TT cells)
    Specification
    BK virus infected 293TT cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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