Anti-SV40 VP2 + VP3 antibody (ab53983)
Key features and details
- Rabbit polyclonal to SV40 VP2 + VP3
- Suitable for: WB
- Reacts with: Simian Virus 40
- Isotype: IgG
Overview
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Product name
Anti-SV40 VP2 + VP3 antibody -
Description
Rabbit polyclonal to SV40 VP2 + VP3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Simian Virus 40 -
Immunogen
Recombinant fusion protein: MAVDLYRPDD YYDILFPGVQ TFVHSVQYLD PRHWGPTLFN AISQAFWRVI QNDIPRLTSQ ELERRTQRYL RDSLARFLEE TTWTVINAPV NWYNSLQDYY STLSPIRPTM VRQVANREGL QISFGHTYDN IDEADSIQQV TERWEAQSQS PNVQSGEFIE KFEAPGGANQ RTAPQWMLPL LLGLYGSVTS ALKAYEDGPN KKKRKLSRGS SQKTKGTSAS AKARHKRRNR SSRS
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General notes
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: Whole serum, 50% Glycerol -
Concentration information loading...
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Purity
Whole antiserum -
Primary antibody notes
This antibody recognises two of the SV40 capsid proteins - VP2 and VP3 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 38,26 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 38,26 kDa. |
Target
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Relevance
There are three simian virus 40 (SV40) capsid proteins - VP1, VP2 and VP3. VP1 assembles into pentamers which have a central cavity that contains a copy of one of the other capsid proteins, VP2 or VP3. This complex is then imported into the cell nucleus. Here, 72 of the complexes assemble around the newly synthesised viral genome to form the icosahedral capsid. -
Database links
- SwissProt: P03093 Simian Virus 40
Images
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All lanes : Anti-SV40 VP2 + VP3 antibody (ab53983) at 1/5000 dilution
Lane 1 : BK virus infected 293T cell lysate
Lane 2 : Mock 293T cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38,26 kDa
Observed band size: 27,38 kDa why is the actual band size different from the predicted?
Exposure time: 6 minutes
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SV40 was ultracentrifuged on CsCl-gradient, fractions were collected from the top of the tube, run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fractions 3,4 and 6 were run on PAGE, and the gel was stained with Coumassie. Equal amounts of SV40 from fraction 3,4 and 6 were run on PAGe and analysed by Western blot with α-VP1 and α-VP2/3
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab53983 has been referenced in 20 publications.
- Spriggs CC et al. Components of the LINC and NPC complexes coordinately target and translocate a virus into the nucleus to promote infection. PLoS Pathog 18:e1010824 (2022). PubMed: 36067270
- Bagchi P et al. Lunapark-dependent formation of a virus-induced ER exit site contains multi-tubular ER junctions that promote viral ER-to-cytosol escape. Cell Rep 37:110077 (2021). PubMed: 34879280
- Spriggs CC et al. Golgi-associated BICD adaptors couple ER membrane penetration and disassembly of a viral cargo. J Cell Biol 219:N/A (2020). PubMed: 32259203
- Panou MM et al. Glibenclamide inhibits BK polyomavirus infection in kidney cells through CFTR blockade. Antiviral Res 178:104778 (2020). PubMed: 32229236
- Chen YJ et al. Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes. J Cell Biol 219:N/A (2020). PubMed: 31895406