Anti-SVCT1 antibody (ab236878)
Key features and details
- Rabbit polyclonal to SVCT1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SVCT1 antibody -
Description
Rabbit polyclonal to SVCT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human SVCT1 aa 173-259.
Database link: Q9UHI7 -
Positive control
- WB: A549 whole cell lysate; Mouse kidney and liver tissue lysate. IHC-P: Human small intestine tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/2000. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Sodium/ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na(+) for each ascorbate. -
Tissue specificity
Highly expressed in adult small intestine, kidney, thymus, ovary, colon, prostate and liver, and in fetal kidney, liver and thymus. -
Sequence similarities
Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 9963 Human
- Entrez Gene: 20522 Mouse
- Omim: 603790 Human
- SwissProt: Q9UHI7 Human
- SwissProt: Q9Z2J0 Mouse
- Unigene: 643467 Human
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Alternative names
- hSVCT1 antibody
- Na(+)/L-ascorbic acid transporter 1 antibody
- S23A1_HUMAN antibody
see all
Images
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All lanes : Anti-SVCT1 antibody (ab236878) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution -
Paraffin-embedded human small intestine tissue stained for SVCT1 with ab236878 at a 1/100 dilution in immunohistochemical analysis.
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A549 (Human lung carcinoma cell line) cells stained for SVCT1 (Green) using ab236878 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236878 has been referenced in 1 publication.
- Ni Y et al. Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation. Int J Biol Sci 18:5168-5184 (2022). PubMed: 35982894