Key features and details
- Rabbit polyclonal to SWAP70 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SWAP70 antibody - C-terminal
See all SWAP70 primary antibodies
DescriptionRabbit polyclonal to SWAP70 - C-terminal
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rhesus monkey
Recombinant fragment within Human SWAP70 (C terminal). The exact sequence is proprietary.
Database link: Q9UH65
- IHC-P: Mouse spleen tissue. ICC/IF: SK-N-SH cells. WB: Jurkat, Raji, NCI-H1299, HEK-293T, A431, HeLa and HepG2 whole cell extracts.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- Jurkat whole cell lysate (ab30128)
- A431 whole cell lysate (ab30132)
- Jurkat whole cell lysate (ab7899)
- A431 whole cell lysate (ab7909)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab228846 in the following tested applications.
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
|WB||1/5000 - 1/20000. Predicted molecular weight: 69 kDa.|
FunctionPhosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
Tissue specificityExpressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
Sequence similaritiesContains 1 PH domain.
DomainThe PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
- Information by UniProt
- KIAA0640 antibody
- 70kDa antibody
- AV235546 antibody
All lanes : Anti-SWAP70 antibody - C-terminal (ab228846) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extracts
Lane 3 : NCI-H1299 (human lung carcinoma cell line) whole cell extracts
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 5 : A431 (human epidermoid carcinoma cell line) whole cell extracts
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
SK-N-SH (human neuroblastoma cell line) cells stained for SWAP70 (green) using ab228846 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
Paraffin-embedded mouse spleen tissue stained for SWAP70 with ab228846 at 1/500 dilution in immunohistochemical analysis.
ab228846 has not yet been referenced specifically in any publications.