Overview

  • Product name

    Anti-SWAP70 antibody - N-terminal
    See all SWAP70 primary antibodies
  • Description

    Rabbit polyclonal to SWAP70 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SWAP70 (N terminal). The exact sequence is proprietary.
    Database link: Q9UH65

  • Positive control

    • WB: HEK-293T, A431, HeLa, HepG2 and Raji whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 69 kDa.

Target

  • Function

    Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.
  • Tissue specificity

    Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
  • Sequence similarities

    Contains 1 PH domain.
  • Domain

    The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
  • Post-translational
    modifications

    Tyrosine-phosphorylated.
  • Cellular localization

    Cytoplasm. Cell membrane. Nucleus. Cell projection > lamellipodium. In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0640 antibody
    • 70kDa antibody
    • AV235546 antibody
    • FLJ39540 antibody
    • HSPC321 antibody
    • SWA 70 protein antibody
    • SWAP 70 antibody
    • SWAP switching B-cell complex 70kDa subunit antibody
    • SWAP-70 antibody
    • SWAP70 antibody
    • Switch associated protein 70 antibody
    • Switch-associated protein 70 antibody
    • SWP70_HUMAN antibody
    see all

Images

  • All lanes : Anti-SWAP70 antibody - N-terminal (ab229814) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS-PAGE gel.

  • Anti-SWAP70 antibody - N-terminal (ab229814) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 69 kDa



    7.5% SDS-PAGE gel.

References

ab229814 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229814.
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