Product nameSWAP70 overexpression 293T lysate (whole cell)
ab94234 is a 293T cell transfected lysate in which Human SWAP70 has been transiently over-expressed using a pCMV-SWAP70 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. Tissue specificity: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760). Similarity: Contains 1 PH domain. Domain: The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding. PTM: Tyrosine-phosphorylated.
Our Abpromise guarantee covers the use of ab94234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94234 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-SWAP70 antibody (ab89532) at 1/500 dilution
Lane 1 :
SWAP70 overexpression 293T lysate (whole cell) (ab94234)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94234 has not yet been referenced specifically in any publications.