Key features and details
- Rabbit polyclonal to SWI5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SWI5 antibody
DescriptionRabbit polyclonal to SWI5
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- ICC/IF: MCF7 cells. WB: Mouse heart, liver, spleen, lung, kidney, brain and stomach tissue lysates. IHC-P: Human colon cancer and kidney tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- Mouse stomach normal tissue lysate - total protein (ab29256)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse liver tissue lysate - total protein (ab29301)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse heart normal tissue lysate - total protein (ab30291)
- Mouse brain tissue lysate - total protein (ab4022)
- Mouse spleen tissue lysate - total protein (ab7937)
Our Abpromise guarantee covers the use of ab234870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 27 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionComponent of the SWI5-SFR1 complex, a complex required for double-strand break repair via homologous recombination.
Sequence similaritiesBelongs to the SWI5/SAE3 family.
- Information by UniProt
- C9orf119 antibody
- DNA repair protein SWI5 homolog antibody
- HBV DNAPTP1-transactivated protein A antibody
MCF7 (human breast adenocarcinoma cell line) cells stained for SWI5 using ab234870 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human colon cancer tissue stained for SWI5 with ab234870 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-SWI5 antibody (ab234870) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Mouse kidney tissue lysate
Lane 6 : Mouse brain tissue lysate
Lane 7 : Mouse stomach tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27 kDa
Paraffin-embedded human kidney tissue stained for SWI5 with ab234870 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234870 has not yet been referenced specifically in any publications.