Overview

  • Product name

    Anti-SWI5 antibody
  • Description

    Rabbit polyclonal to SWI5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human SWI5 aa 54-112.
    Sequence:

    PVHLRGRSPTPALDPLAPLNPLIRGPRTPGLRRWIQSLALLLPNCSSSRI PTVPRPHSG


    Database link: Q1ZZU3

  • Positive control

    • ICC/IF: MCF7 cells. WB: Mouse heart, liver, spleen, lung, kidney, brain and stomach tissue lysates. IHC-P: Human colon cancer and kidney tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab234870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

Images

  • MCF7 (human breast adenocarcinoma cell line) cells stained for SWI5 using ab234870 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human colon cancer tissue stained for SWI5 with ab234870 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-SWI5 antibody (ab234870) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Mouse lung tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Mouse brain tissue lysate
    Lane 7 : Mouse stomach tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 27 kDa

  • Paraffin-embedded human kidney tissue stained for SWI5 with ab234870 at 1/100 dilution in immunohistochemical analysis.

References

ab234870 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab234870.
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