Overview

  • Product name

    Swine Haptoglobin ELISA Kit
    See all Haptoglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 1 20 5.4%
    Plasma 2 20 4.9%
    Plasma 3 20 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 1 20 9.9%
    Plasma 2 20 9.5%
    Plasma 3 20 10.1%
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.55 ng/ml
  • Range

    1.875 ng/ml - 120 ng/ml
  • Recovery

    97 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Pig
    Does not react with: Mouse, Rat, Rabbit, Cow, Human, Monkey
  • Product overview

    The Swine Haptoglobin ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab137979) is designed for detection of swine haptoglobin in plasma, serum, urine, and cell culture samples.


    This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine haptoglobin in approximately 4 hours. A polyclonal antibody specific for swine haptoglobin has been pre-coated onto a 96-well microplate with removable strips. Swine haptoglobin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for swine haptoglobin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Haptoglobin (HP, zonulin) is a plasma protein with hemoglobin-binding capacity and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    80x Biotinylated Swine Haptoglobin 1 x 75µl
    Anti-Swine Haptoglobin coated Microplate (12 x 8 wells) 1 unit
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
    Swine Haptoglobin Standard 1 vial
  • Research areas

  • Function

    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Anhaptoglobinemia
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Binding peptide
    • BP
    • Haptoglobin alpha chain
    • Haptoglobin alpha(1S) beta
    • Haptoglobin alpha(2FS) beta
    • Haptoglobin beta chain
    • Haptoglobin, alpha polypeptide
    • Haptoglobin, beta polypeptide
    • HP
    • HP2 ALPHA2
    • HP2ALPHA2
    • HPA1S
    • HPT
    • HPT_HUMAN
    • MGC111141
    • Zonulin
    see all

Applications

Our Abpromise guarantee covers the use of ab223136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of Swine Haptoglobin standard curve in 1X Diluent N. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab223136 has not yet been referenced specifically in any publications.

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