Key features and details
- Sensitivity: 0.55 ng/ml
- Range: 1.875 ng/ml - 120 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Product nameSwine Haptoglobin ELISA Kit
See all Haptoglobin kits
Intra-assay Sample n Mean SD CV% Plasma 1 20 5.4% Plasma 2 20 4.9% Plasma 3 20 5.6% Inter-assay Sample n Mean SD CV% Plasma 1 20 9.9% Plasma 2 20 9.5% Plasma 3 20 10.1%
Sample typeCell culture supernatant, Urine, Serum, Plasma
Assay typeSandwich (quantitative)
Range1.875 ng/ml - 120 ng/ml
Assay time4h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Pig
Does not react with: Mouse, Rat, Rabbit, Cow, Human, Monkey
The Swine Haptoglobin ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab137979) is designed for detection of swine haptoglobin in plasma, serum, urine, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures swine haptoglobin in approximately 4 hours. A polyclonal antibody specific for swine haptoglobin has been pre-coated onto a 96-well microplate with removable strips. Swine haptoglobin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for swine haptoglobin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Haptoglobin (HP, zonulin) is a plasma protein with hemoglobin-binding capacity and a plasma glycoprotein that forms a stable complex with hemoglobin to aid the recycling of heme iron.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 80x Biotinylated Swine Haptoglobin 1 x 75µl Anti-Swine Haptoglobin coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Swine Haptoglobin Standard 1 vial
FunctionAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
Tissue specificityExpressed by the liver and secreted in plasma.
Involvement in diseaseAnhaptoglobinemia
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
- Information by UniProt
- Binding peptide
- Haptoglobin alpha chain
ab223136 has not yet been referenced specifically in any publications.