Overview

  • Product name

    Anti-Swine Influenza A Hemagglutinin antibody
    See all Swine Influenza A Hemagglutinin primary antibodies
  • Description

    Rabbit polyclonal to Swine Influenza A Hemagglutinin
  • Host species

    Rabbit
  • Specificity

    Specific for the Swine Influenza A (H1N1) Hemagglutinin. Does not bind the corresponding Hemagglutinin from seasonal H1N1 influenza virus.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Influenza A
  • Immunogen

    Synthetic peptide containing the sequence specific to Swine Influenza A (H1N1) Hemagglutinin (ACQ76314).

Properties

Applications

Our Abpromise guarantee covers the use of ab91641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 1 - 2 µg/ml.
    WB: Use at a concentration of 2 µg/ml. Predicted molecular weight: 61 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication.
    • Database links

      • Alternative names

        • Swine-Origin Influenza A (H1N1) Hemagglutinin: S-OIV A Hemagglutinin, Swine flu H1, A antibody

      Images

      • All lanes : Anti-Swine Influenza A Hemagglutinin antibody (ab91641) at 2 µg/ml

        Lane 1 : Recombinant seasonal influenza Hemagglutinin
        Lane 2 : Recombinant Swine Influenza A (H1N1) Hemagglutinin

        Predicted band size: 61 kDa

      References

      This product has been referenced in:

      • Hussain S  et al. Mutation of Influenza A Virus PA-X Decreases Pathogenicity in Chicken Embryos and Can Increase the Yield of Reassortant Candidate Vaccine Viruses. J Virol 93:N/A (2019). Read more (PubMed: 30381488) »
      See 1 Publication for this product

      Customer reviews and Q&As

      1-4 of 4 Abreviews or Q&A

      Answer



      I've confirmed with the lab that this antibody was raised against A/California/14/09, which shares 99% overall homology to A/California/04/09. The specific immunogen of the antibody ab91641 only shares 92% homology with A/California/04/09 however.

      Read More

      Answer

      Thank you for your reply.

      I am sorry to hear that the new vial also did not work. I'm sending a vial of ab91643, however I can't guarantee that it will work in WB. We recommend using it at 1:1000 for ELISA, so for Western blot I would recommend using at least 1:500 (2 ug/mL) as long as the titers in your sample are high.

      This vial should be arriving within a couple of days on the order ***. Please keep me updated about the results with this antibody, and let me know if you have any questions or if there is anything else that we can do for you.

      Read More

      Answer

      Thank you for your reply, and you're very welcome.

      Please keep me updated about any new results with the new vial and protocol alterations. I'vetracked the shipment,and the vial should arrive today.

      I look forward to hearing from you. Let me know if you have any questions or if there is anything else that we can do. Have a great day.

      Read More

      Question
      Answer

      Thank you for your call last week and for letting us know about the trouble with ab91641.

      I aligned the hemagglutinin protein from the strain of H1N1 that the antibody was raised against(UniProt accession ACQ76314) with the hemagglutinin protein from the strain that you are working with (EMBL accession FJ998208), and these proteins are 99% identical across the full sequences. So, this antibody should be able toreact with your samples.

      I received the full protocol used to test this antibody from the lab, and there are some suggestions that may be helpful. We used 5% skim milk in TBS as a blocking buffer, so you may want to try this instead of the commercial block. You can also try a higher concentration. as 2 ug/mL was used against a sample of recombinant protein. I would recommend up to 1:250 and an overnight incubationto see if any specific signal can be detected.

      I hope this information will be useful, but as we discussed, I would also be happy to send a replacement antibody or issue a credit or refund if you prefer. If you do decide to try these protocol alterations, please keep me updated about the results, and I will be happy to help you further if necessary.

      Please let me know if you have any questions or if there is anything else that we can do for you.

      Read More

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