Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195)
Key features and details
- Mouse monoclonal [158.2] to SYBL1/VAMP-7
- Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SYBL1/VAMP-7 antibody [158.2]
See all SYBL1/VAMP-7 primary antibodies -
Description
Mouse monoclonal [158.2] to SYBL1/VAMP-7 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Dog -
Immunogen
Recombinant full length protein corresponding to Human SYBL1/VAMP-7.
Database link: P51809 -
Positive control
- extract from rat brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
158.2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 19138172
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Flow Cyt |
1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/2000. Predicted molecular weight: 24 kDa.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 19138172 |
Flow Cyt
1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 24 kDa. |
Target
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Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. -
Tissue specificity
Detected in all tissues tested. -
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 longin domain.
Contains 1 v-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle membrane. Golgi apparatus > trans-Golgi network membrane. Late endosome membrane. Lysosome membrane. Endoplasmic reticulum membrane. Cytoplasmic vesicle > phagosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 6845 Human
- Entrez Gene: 20955 Mouse
- Entrez Gene: 85491 Rat
- Omim: 300053 Human
- SwissProt: P51809 Human
- SwissProt: P70280 Mouse
- SwissProt: Q9JHW5 Rat
- Unigene: 24167 Human
see all -
Alternative names
- FLJ53045 antibody
- FLJ53762 antibody
- FLJ54296 antibody
see all
Images
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Anti-SYBL1/VAMP-7 antibody [158.2] (ab36195) at 1/500 dilution + Tissue lysates prepared from rat brain
Predicted band size: 24 kDa -
Overlay histogram showing PC12 cells stained with ab36195 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36195, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab36195 has been referenced in 25 publications.
- West ZE et al. The trans-SNARE complex VAMP4/Stx6/Stx7/Vti1b is a key regulator of Golgi to late endosome MT1-MMP transport in macrophages. Traffic 22:368-376 (2021). PubMed: 34476885
- Hülsemann M et al. TC10 regulates breast cancer invasion and metastasis by controlling membrane type-1 matrix metalloproteinase at invadopodia. Commun Biol 4:1091 (2021). PubMed: 34531530
- Tian X et al. DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B. Autophagy 16:797-810 (2020). PubMed: 31251111
- Liu Y et al. Ablation of All Synaptobrevin vSNAREs Blocks Evoked But Not Spontaneous Neurotransmitter Release at Neuromuscular Synapses. J Neurosci 39:6049-6066 (2019). PubMed: 31160536
- Röhl J et al. Invasion by activated macrophages requires delivery of nascent membrane-type-1 matrix metalloproteinase through late endosomes/lysosomes to the cell surface. Traffic 20:661-673 (2019). PubMed: 31297933