For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    syk-antibody-ab155187.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Platelets
Share by email

Anti-Syk antibody (ab155187)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Syk antibody (ab155187)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
  • Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody (ab155187)
  • Immunoprecipitation - Anti-Syk antibody (ab155187)
  • Western blot - Anti-Syk antibody (ab155187)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
  • Western blot - Anti-Syk antibody (ab155187)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
  • Western blot - Anti-Syk antibody (ab155187)

Key features and details

  • Rabbit polyclonal to Syk
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-CD4 antibody [EPR6855] (ab133616)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human Syk protein (ab60886)

View more associated products

Overview

  • Product name

    Anti-Syk antibody
    See all Syk primary antibodies
  • Description

    Rabbit polyclonal to Syk
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 148-483 of Human Syk (UniProt P43405).

  • Positive control

    • WB: BMDM, Raji, A431, mouse spleen tissue; IHC-P: Human cervical cancer, endometrium; rat spleen tissue; IF: A431 whole cell lysate; IP: A431 whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Immunology
    • Innate Immunity
    • Mast Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant human Syk protein (ab60886)
  • Related Products

    • Recombinant human Syk protein (ab60886)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
IP
1/100 - 1/500.

Target

  • Function

    Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigen receptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays also a role in T-cell receptor signaling. Plays also a crucial role in the innate immune response to fungal, bacterial and viral pathogens. It is for instance activated by the membrane lectin CLEC7A. Upon stimulation by fungal proteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence of pathogens. Regulates neutrophil degranulation and phagocytosis through activation of the MAPK signaling cascade. Also mediates the activation of dendritic cells by cell necrosis stimuli. Also involved in mast cells activation. Also functions downstream of receptors mediating cell adhesion. Relays for instance, integrin-mediated neutrophils and macrophages activation and P-selectin receptor/SELPG-mediated recruitment of leukocytes to inflammatory loci. Plays also a role in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphatic vascular separation. It is also required for osteoclast development and function. Functions in the activation of platelets by collagen, mediating PLCG2 phosphorylation and activation. May be coupled to the collagen receptor by the ITAM domain-containing FCER1G. Also activated by the membrane lectin CLEC1B that is required for activation of platelets by PDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engaged by fibrinogen.
  • Tissue specificity

    Widely expressed in hematopoietic cells (at protein level). Within the B-cells compartment it is for instance expressed for pro-B-cells to plasma cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.
  • Domain

    The SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins. Some proteins like CLEC1B have a partial ITAM domain (also called hemITAM) containing a single YxxL motif. The interaction with SYK requires CLEC1B homodimerization.
  • Post-translational
    modifications

    Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.
    Autophosphorylated. Phosphorylated on tyrosine residues by LYN following receptors engagement. Phosphorylation on Tyr-323 creates a binding site for CBL, an adapter protein that serves as a negative regulator of BCR-stimulated calcium ion signaling. Phosphorylation at Tyr-348 creates a binding site for VAV1. Phosphorylation on Tyr-348 and Tyr-352 enhances the phosphorylation and activation of phospholipase C-gamma and the early phase of calcium ion mobilization via a phosphoinositide 3-kinase-independent pathway (By similarity). Phosphorylation on Ser-297 is very common, it peaks 5 minutes after BCR stimulation, and creates a binding site for YWHAG. Phosphorylation at Tyr-630 creates a binding site for BLNK. Dephosphorylated by PTPN6.
  • Cellular localization

    Cell membrane. Cytoplasm, cytosol.
  • Target information above from: UniProt accession P43405 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6850 Human
    • Entrez Gene: 20963 Mouse
    • Entrez Gene: 25155 Rat
    • Omim: 600085 Human
    • SwissProt: P43405 Human
    • SwissProt: P48025 Mouse
    • SwissProt: Q64725 Rat
    • Unigene: 371720 Human
    • Unigene: 375031 Mouse
    • Unigene: 87407 Rat
    see all
  • Alternative names

    • EC 2.7.10.2 antibody
    • kinase Syk antibody
    • KSYK antibody
    • KSYK_HUMAN antibody
    • p72-Syk antibody
    • p72syk antibody
    • Spleen tyrosine kinase antibody
    • Syk antibody
    • Tyrosine protein kinase SYK antibody
    • Tyrosine-protein kinase SYK antibody
    see all

Images

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    All lanes : Anti-Syk antibody (ab155187) at 1/1000 dilution

    Lane 1 : BMDM (Mouse bone marrow-derived macrophage) whole cell lysate
    Lane 2 : Syk null cell

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    10% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody (ab155187)
    Immunocytochemistry/ Immunofluorescence - Anti-Syk antibody (ab155187)

    Immunofluorescence analysis of methanol-fixed A431 (human epidermoid carcinoma cell line) cells labeling Syk with ab155187 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

  • Immunoprecipitation - Anti-Syk antibody (ab155187)
    Immunoprecipitation - Anti-Syk antibody (ab155187)

    Immunoprecipitation analysis of SYK protein from A431 (human epidermoid carcinoma cell line) whole cell lysates.

    IP samples:

    1: A431 whole cell lysates;

    2: Control with IgG;

    3: Immunoprecipitate of Syk protein by 5 μg of ab155187.

    Western blot analysis was performed using ab155187, followed by anti-Rabbit IgG antibody.

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    Anti-Syk antibody (ab155187) at 1/2000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    7.5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    Anti-Syk antibody (ab155187) at 1/2000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    7.5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syk antibody (ab155187)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue staining Syk at cytoplasm with ab155187 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Western blot - Anti-Syk antibody (ab155187)
    Western blot - Anti-Syk antibody (ab155187)
    Anti-Syk antibody (ab155187) at 1/1000 dilution + Mouse spleen tissue at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 72 kDa



    7.5% SDS-PAGE

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab155187? Please let us know so that we can cite the reference in this datasheet.

ab155187 has been referenced in 3 publications.

  • Zhang NN  et al. Flavanomarein inhibits high glucose-stimulated epithelial-mesenchymal transition in HK-2 cells via targeting spleen tyrosine kinase. Sci Rep 10:439 (2020). PubMed: 31949205
  • Kan B  et al. Cellular metabolism constrains innate immune responses in early human ontogeny. Nat Commun 9:4822 (2018). PubMed: 30446641
  • Afifiyan N  et al. Over expression of proteins that alter the intracellular signaling pathways in the cytoplasm of the liver cells forming Mallory-Denk bodies. Exp Mol Pathol 102:106-114 (2017). IHC-P ; Human . PubMed: 28089901

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Western blot abreview for Anti-Syk antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
endothelial cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Dr. PeiYi Su

Verified customer

Submitted May 24 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.