Overview

  • Product name

  • Description

    Rabbit polyclonal to SYN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Rat SYN2 aa 52-65 conjugated to keyhole limpet haemocyanin.
    Sequence:

    ASPGPERRPPPAQA

  • Positive control

    • Rat brain tissue extract.
  • General notes

     This product was previously labelled as Synapsin II

     

Properties

Applications

Our Abpromise guarantee covers the use of ab13258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 74, 54 kDa (predicted molecular weight: 57.5 kDa).
IP 1/100.
ICC/IF Use at an assay dependent dilution. PubMed: 19714568

Target

  • Relevance

    Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal.
  • Cellular localization

    Cell junction, synapse.
  • Database links

  • Alternative names

    • SYN 2 antibody
    • SYN II antibody
    • SYN IIa antibody
    • SYN IIb antibody
    • SYN2 antibody
    • Synapsin 2 antibody
    • Synapsin II isoform IIa antibody
    • Synapsin II isoform IIb antibody
    • Synapsin2 antibody
    • SynapsinII antibody
    • SYNII antibody
    • SYNIIa antibody
    • SYNIIb antibody
    see all

Images

  • Anti-SYN2 antibody (ab13258) at 1/2000 dilution + Rat whole brain tissue lysate at 15 µg

    Secondary
    HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57.5 kDa
    Observed band size: 60,78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocked with 5% milk for 1 hour at 25°C.

    Incubated with the primary antibody diluted in TBS + 0.1% Tween for 18 hours at 4°C.

    See Abreview

  • Western blot analysis of rat cerebellum tissue, staining SYN2 with ab13258.

    Samples were immunoprecipitated with antibodies against CB or mGluR4 and co-immunoprecipitated SYN2 was detected using ab13258. Synapsin I was detected using ab8 - Rabbit polyclonal to Synapsin I.

References

This product has been referenced in:

  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). IF . Read more (PubMed: 27592818) »
  • Ramos C  et al. Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum. J Biol Chem 287:20176-86 (2012). WB ; Rat . Read more (PubMed: 22528491) »
See all 6 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (whole brain)
Gel Running Conditions
Reduced Denaturing (7.5% Tris gel)
Loading amount
15 µg
Specification
whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 01 2015

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