Recombinant
RabMAb

Recombinant Anti-SYN2 antibody [EPR3277] (ab76494)

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Overview

  • Product name

    Anti-SYN2 antibody [EPR3277]
    See all SYN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3277] to SYN2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SYN2 aa 500-600. The exact sequence is proprietary.
    (Peptide available as ab177842)

  • Positive control

    • WB: fetal brain cell lysate; IHC-P: human brain tissue;

  • General notes

     

     

     This product was previously labelled as Synapsin II

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Relevance

      Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal.

    • Cellular localization

      Cell junction, synapse.

    • Database links

      see all

    • Alternative names

      • SYN 2 antibody
      • SYN II antibody
      • SYN IIa antibody
      • SYN IIb antibody
      • SYN2 antibody
      • Synapsin 2 antibody
      • Synapsin II isoform IIa antibody
      • Synapsin II isoform IIb antibody
      • Synapsin2 antibody
      • SynapsinII antibody
      • SYNII antibody
      • SYNIIa antibody
      • SYNIIb antibody
      see all

    Images

    • Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)
      Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)
      Anti-SYN2 antibody [EPR3277] (ab76494) at 1/20000 dilution + fetal
      brain cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 63 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)

      Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/100 ab76494

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Kim JH  et al. Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields. Sci Rep 9:1201 (2019). Read more (PubMed: 30718744) »
    • Cicvaric A  et al. Sustained consumption of cocoa-based dark chocolate enhances seizure-like events in the mouse hippocampus. Food Funct N/A:N/A (2018). Read more (PubMed: 29431797) »
    See all 5 Publications for this product

    Publishing research using ab76494? Please let us know so that we can cite the reference in this datasheet.

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