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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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RecombinantRabMAb

Recombinant Anti-SYN2 antibody [EPR3277] (ab76494)

  • Datasheet
  • SDS
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Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)
  • Anti-SYN2 antibody [EPR3277] (ab76494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3277] to SYN2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Protein
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Recombinant Human SYN2 protein (ab114589)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-SYN2 antibody [EPR3277]
    See all SYN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3277] to SYN2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human SYN2 aa 500-600. The exact sequence is proprietary.
    (Peptide available as ab177842)

  • Positive control

    • WB: fetal brain cell lysate; IHC-P: human brain tissue;
  • General notes

     

     

     This product was previously labelled as Synapsin II

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3277
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Associated products

  • Alternative Versions

    • Anti-SYN2 antibody [EPR3277] - BSA and Azide free (ab247480)
  • Immunizing Peptide (Blocking)

    • SYN2 peptide (ab177842)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SYN2 protein (ab114589)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Relevance

      Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal.
    • Cellular localization

      Cell junction, synapse.
    • Database links

      • Entrez Gene: 6854 Human
      • Entrez Gene: 20965 Mouse
      • Entrez Gene: 29179 Rat
      • Omim: 600755 Human
      • SwissProt: Q92777 Human
      • SwissProt: Q64332 Mouse
      • SwissProt: Q63537 Rat
      • Unigene: 445503 Human
      • Unigene: 506 Rat
      see all
    • Alternative names

      • SYN 2 antibody
      • SYN II antibody
      • SYN IIa antibody
      • SYN IIb antibody
      • SYN2 antibody
      • Synapsin 2 antibody
      • Synapsin II isoform IIa antibody
      • Synapsin II isoform IIb antibody
      • Synapsin2 antibody
      • SynapsinII antibody
      • SYNII antibody
      • SYNIIa antibody
      • SYNIIb antibody
      see all

    Images

    • Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)
      Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)
      Anti-SYN2 antibody [EPR3277] (ab76494) at 1/20000 dilution + fetal
      brain cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 63 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)

      Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/100 ab76494

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-SYN2 antibody [EPR3277] (ab76494)
      Anti-SYN2 antibody [EPR3277] (ab76494)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab76494? Please let us know so that we can cite the reference in this datasheet.

    ab76494 has been referenced in 5 publications.

    • Kim JH  et al. Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields. Sci Rep 9:1201 (2019). PubMed: 30718744
    • Cicvaric A  et al. Sustained consumption of cocoa-based dark chocolate enhances seizure-like events in the mouse hippocampus. Food Funct N/A:N/A (2018). PubMed: 29431797
    • Kim JH  et al. Changes in numbers and size of synaptic vesicles of cortical neurons induced by exposure to 835 MHz radiofrequency-electromagnetic field. PLoS One 12:e0186416 (2017). PubMed: 29045446
    • Wei H  et al. Diffuse and specific tectopulvinar terminals in the tree shrew: synapses, synapsins, and synaptic potentials. PLoS One 6:e23781 (2011). IHC (PFA fixed) . PubMed: 21858222
    • Agostini M  et al. Neuronal differentiation by TAp73 is mediated by microRNA-34a regulation of synaptic protein targets. Proc Natl Acad Sci U S A 108:21093-8 (2011). WB . PubMed: 22160687

    Customer reviews and Q&As

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