Recombinant Anti-SYN2 antibody [EPR3277] (ab76494)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-SYN2 antibody [EPR3277]
See all SYN2 primary antibodies -
Description
Rabbit monoclonal [EPR3277] to SYN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SYN2 aa 500-600. The exact sequence is proprietary.
(Peptide available asab177842) -
Positive control
- WB: fetal brain cell lysate; IHC-P: human brain tissue;
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General notes
This product was previously labelled as Synapsin II
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3277 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Relevance
Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. -
Cellular localization
Cell junction, synapse. -
Database links
- Entrez Gene: 6854 Human
- Entrez Gene: 20965 Mouse
- Entrez Gene: 29179 Rat
- Omim: 600755 Human
- SwissProt: Q92777 Human
- SwissProt: Q64332 Mouse
- SwissProt: Q63537 Rat
- Unigene: 445503 Human
see all -
Alternative names
- SYN 2 antibody
- SYN II antibody
- SYN IIa antibody
see all
Images
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![Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)](https://www.abcam.com/ps/products/76/ab76494/Images/ab76494-91939-anti-synapsin-ii-antibody-epr3277-western-blot.jpg)
Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)Anti-SYN2 antibody [EPR3277] (ab76494) at 1/20000 dilution + fetal
brain cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)](https://www.abcam.com/ps/products/76/ab76494/Images/ab76494-91938-anti-synapsin-ii-antibody-epr3277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/100 ab76494
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
References
This product has been referenced in:
- Kim JH et al. Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields. Sci Rep 9:1201 (2019). Read more (PubMed: 30718744) »
- Cicvaric A et al. Sustained consumption of cocoa-based dark chocolate enhances seizure-like events in the mouse hippocampus. Food Funct N/A:N/A (2018). Read more (PubMed: 29431797) »
