Recombinant Anti-SYN2 antibody [EPR3277] (ab76494)
![Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)](https://www.abcam.com/ps/products/76/ab76494/Images/ab76494-91939-anti-synapsin-ii-antibody-epr3277-western-blot.jpg "Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3277] to SYN2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SYN2 antibody [EPR3277]
See all SYN2 primary antibodies -
Description
Rabbit monoclonal [EPR3277] to SYN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human SYN2 aa 500-600. The exact sequence is proprietary.
(Peptide available asab177842) -
Positive control
- WB: fetal brain cell lysate; IHC-P: human brain tissue;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3277 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842).
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.
Target
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Function
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons. -
Tissue specificity
Central and peripheral nervous systems. -
Sequence similarities
Belongs to the synapsin family. -
Domain
The A region binds phospholipids with a preference for negatively charged species. -
Post-translational
modificationsPhosphorylation at Ser-10 dissociates synapsins from synaptic vesicles. Phosphorylation at Ser-425 by MAPK1/ERK2 and/or MAPK3/ERK1 may play a role in noradrenaline secretion by sympathetic neurons. -
Cellular localization
Cell junction, synapse. - Information by UniProt
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Database links
- Entrez Gene: 6854 Human
- Entrez Gene: 20965 Mouse
- Entrez Gene: 29179 Rat
- Omim: 600755 Human
- SwissProt: Q92777 Human
- SwissProt: Q64332 Mouse
- SwissProt: Q63537 Rat
- Unigene: 445503 Human
see all -
Alternative names
- SYN 2 antibody
- SYN II antibody
- SYN IIa antibody
see all
Images
-
Western blot - Anti-SYN2 antibody [EPR3277] (ab76494)Anti-SYN2 antibody [EPR3277] (ab76494) at 1/20000 dilution + fetal
brain cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)](https://www.abcam.com/ps/products/76/ab76494/Images/ab76494-91938-anti-synapsin-ii-antibody-epr3277-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SYN2 antibody [EPR3277] (ab76494)Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/100 ab76494
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-SYN2 antibody [EPR3277] (ab76494)](https://www.abcam.com/ps/products/76/ab76494/Images/ab76494-4-benefits-of-recombinant-antibodies.png)
Anti-SYN2 antibody [EPR3277] (ab76494)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab76494 has been referenced in 5 publications.
- Kim JH et al. Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields. Sci Rep 9:1201 (2019). PubMed: 30718744
- Cicvaric A et al. Sustained consumption of cocoa-based dark chocolate enhances seizure-like events in the mouse hippocampus. Food Funct N/A:N/A (2018). PubMed: 29431797
- Kim JH et al. Changes in numbers and size of synaptic vesicles of cortical neurons induced by exposure to 835 MHz radiofrequency-electromagnetic field. PLoS One 12:e0186416 (2017). PubMed: 29045446
- Wei H et al. Diffuse and specific tectopulvinar terminals in the tree shrew: synapses, synapsins, and synaptic potentials. PLoS One 6:e23781 (2011). IHC (PFA fixed) . PubMed: 21858222
- Agostini M et al. Neuronal differentiation by TAp73 is mediated by microRNA-34a regulation of synaptic protein targets. Proc Natl Acad Sci U S A 108:21093-8 (2011). WB . PubMed: 22160687