Overview

  • Product name
  • Description
    Rabbit polyclonal to Synapsin I
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Synapsin I. Corresponds to residues surrounding amino acid 12 of Synapsin I.

  • Positive control
    • WB: Human, mouse and rat brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab18814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 74 kDa).
IHC-FoFr Use at an assay dependent concentration. PubMed: 22579680

Target

  • Function
    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • Involvement in disease
    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • Sequence similarities
    Belongs to the synapsin family.
  • Post-translational
    modifications
    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

Images

  • All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

    Lane 1 : Human brain whole cell lysate - untreated
    Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

  • All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

    Lane 1 : Mouse brain whole cell lysate - untreated
    Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

  • All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution

    Lane 1 : Rat brain whole cell lysate - untreated
    Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).

  • Anti-Synapsin I antibody (ab18814) at 1 µg/ml + Mouse brain tissue lysate.

    Predicted band size: 74 kDa
    Observed band size: ~77 kDa why is the actual band size different from the predicted?



    Secondary antibody - goat anti-rabbit HRP (ab6721)

References

This product has been referenced in:
  • Zhang Y  et al. Clostridium difficile toxin B recombinant protein inhibits tumor growth and induces apoptosis through inhibiting Bcl-2 expression, triggering inflammatory responses and activating C-erbB-2 and Cox-2 expression in breast cancer mouse model. Biomed Pharmacother 101:391-398 (2018). Read more (PubMed: 29501042) »
  • Schrod N  et al. Pleomorphic linkers as ubiquitous structural organizers of vesicles in axons. PLoS One 13:e0197886 (2018). Read more (PubMed: 29864134) »
See all 7 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Loading amount
2 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Rat Tissue lysate - whole (hippocampus tissue lysate)
Specification
hippocampus tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Jun 18 2014

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification
Hippocampal homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Sunetra Jitkar

Verified customer

Submitted Feb 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain)
Loading amount
20 µg
Specification
Whole brain
Gel Running Conditions
Reduced Denaturing (gel 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 22 2012

Answer

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement. Ab64581 has been tested in IHC and reacts with mouse, and has very positive reviews. I've included a link to the datasheet below so you can take a look and make sure this is the one you want. I'm happy to send you a vial free of charge.

For ab18814, this product is produced by a third party so we do not have the exact immunogen sequence. We do know that the immunogen peptide is taken from around amino acid 12. In my experience, immunogen peptides are normallysomewhere around 15-20 amino acids long, but obviously this could be different for this antibody. I'm going to see how much information I can get from the originator of the antibody, but I am not sure whether they will release the exact sequence to us.

Please let me know if you'd like a vial of ab64581 and I will set the order up for you.

I look forward to your reply. Have a great day!

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (hippocampus)
Loading amount
5 µg
Specification
hippocampus
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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