Anti-Synapsin I antibody (ab8)

Thank you for your patience. The IF validation for this antibody was done by a collaborator, so unfortunately we do not have a specific protocol to provide. The neurons were cultured for 14 days. I hope this helps, please let me know if you need any additional information or assistance.

Thank you for your call yesterday and for letting us know about the trouble with ab8 in IP.
I've attached the specific IP protocol for ab8, which was optimized with Sacc cells. I am hopeful that this protocol will be a good referece, but if you would like to send some details about your experiment I will also see if there are any other suggestions to make.
Please keep me updated about the results using the replacement vial, and I let me know if you have any questions or if there is anything else that I can do for you.

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement. Ab64581 has been tested in IHC and reacts with mouse, and has very positive reviews. I've included a link to the datasheet below so you can take a look and make sure this is the one you want. I'm happy to send you a vial free of charge.

For ab18814, this product is produced by a third party so we do not have the exact immunogen sequence. We do know that the immunogen peptide is taken from around amino acid 12. In my experience, immunogen peptides are normallysomewhere around 15-20 amino acids long, but obviously this could be different for this antibody. I'm going to see how much information I can get from the originator of the antibody, but I am not sure whether they will release the exact sequence to us.

Please let me know if you'd like a vial of ab64581 and I will set the order up for you.

I look forward to your reply. Have a great day!

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab18258 with the order number 996056. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Thanks for your reply. One example of a popular target for synapse staining is PSD95. A good antibody to detect this protein in a variety of applications and species is ab18258. I would be happy to send you a vial of ab18258 if you are interested. Please check the online datasheet for this product at the link below to make sure it is tested in the applications and species you are working with: https://www.abcam.com/PSD95-antibody-Synaptic-Marker-ab18258.html I hope this is helpful. I look forward to hearing from you.

Thank you for your enquiry. It seems that we had an error in our datasheet. The recommended dilution for WB should read as "WB: 1/1000". Please rest assure that the datasheet has already been updated to show the changes. Thank you for informing us about this and I sincerely apologize for the inconvenience. Should you require any further assistance, please do not hesitate to contact us.

I'm sorry to hear that you are having trouble getting this antibody to work in your research. The antibody is suitable for use in IHC on both frozen and fixed sections and Novus recommend a dilution of 1:1000. There is a reference on the datasheet that features the use of this antibody, but I'm not sure whether or not that was in IHC: Danglot L et al. Association of gephyrin with synaptic and extrasynaptic GABAA receptors varies during development in cultured hippocampal neurons. Mol Cell Neurosci 23:264-78 (2003). PubMed: 12812758. In order to help you further I will need more details of your protocol and the nature of the material on which you are working. The technical help section of our website will be useful to you as it contains many helpful suggestions as to how you could optimise your protocol. Go to the Abcam homepage www.abcam.com and type in ab8 into the search box. Once on the datasheet, click through to the enquiries tab and onto the link for technical support. You can specify that you are using the antibody in IHC. If this does not help, please complete the form to tell us more about how you are using the antibody.

Unfortunately, we do not know how they will perform in paraffin-embedded sections. As a reference, Reference #1 uses the same type and source of antibody as we sell (Stone, et al. The Journal of Neuroscience. 14(1): 301-309, 1994).