Anti-Synapsin I antibody - Synaptic Marker (ab64581)
Rabbit polyclonal Synapsin I antibody. Validated in WB, IP, IHC, ICC/IF and tested in Mouse, Rat, Sheep, Goat, Human. Cited in 29 publication(s). Independently reviewed in 12 review(s).
- Datasheet
- References (34)
- Protocols
Overview
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Product name
Anti-Synapsin I antibody - Synaptic Marker
See all Synapsin I primary antibodies -
Description
Rabbit polyclonal to Synapsin I - Synaptic Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-FoFr, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Goat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Rat Synapsin I aa 600-700 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available asab64580) -
Positive control
- WB: Mouse spinal cord, mouse brain and rat brain tissue lysates. IP: Mouse brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab64581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa). Abcam recommends using 3-5% milk as the blocking agent. |
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| IHC-P | Use at an assay dependent concentration. | |
| IHC-FoFr | 1/100. | |
| ICC/IF | 1/200. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
Involvement in disease
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
Sequence similarities
Belongs to the synapsin family. -
Post-translational
modificationsSubstrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
- SwissProt: P09951 Rat
- Unigene: 225936 Human
see all -
Alternative names
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
Images
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Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image is courtesy of an anonymous AbreviewAnti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution + Rat whole brain tissue lysate at 15 µg
Secondary
Donkey anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocked with 5% milk for 1 hour at 25°C.
Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image was taken from an abreview submitted by an Carl Hobbs.IHC-P image of ab64581 staining of Synapsin 1 in lamb cerebellum. Heat mediated antigen retrieval buffer was performed using citric acid buffer. The sections were then incubated in 1% BSA to block non-specific protein-protein interactions. The sections were incubated with ab64581 (1:100) for two hours at +21°C, followed by biotin conjugated secondary antibody. There appears to be appropriate, identical positivity in lamb, goat, mouse and rat brain. -
Immunocytochemistry/ Immunofluorescence - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image is courtesy of an Abreview submitted by Ruma Raha-Chowdhuryab64581 staining cultured rat hippocampal neurons by ICC/IF. The cultured neurons were fixed with methanol, permeabilized with 0.3% Triton X in 0.1% PBS and blocked with 10% donkey serum for 30 minutes at 24°C. The cultured neurons were then stained with ab64581 at 1/200 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with Hoechst and can be observed in blue.
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Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)All lanes : Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?
The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data). -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image is courtesy of an anonymous Abreviewab64581 staining Synapsin I in rat brain (cerebellum) tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.01% Triton X-100, blocked with 10% serum for 2 hours at 25°C; antigen retrieval was by heat mediation in a citrate buffer. The sample was incubated with the primary antibody (1/300) at 25°C for 12 hours. An undiluted Alexa Fluor® 555-conjugated goat anti-rabbit polyclonal was used as the secondary antibody.
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Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.
Protocols
References
This product has been referenced in:
- Zhong Q et al. FCPR16, a novel phosphodiesterase 4 inhibitor, produces an antidepressant-like effect in mice exposed to chronic unpredictable mild stress. Prog Neuropsychopharmacol Biol Psychiatry 90:62-75 (2019). Read more (PubMed: 30391306) »
- Kim JH et al. Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields. Sci Rep 9:1201 (2019). Read more (PubMed: 30718744) »
