Anti-Synapsin I antibody - Synaptic Marker (ab64581)
Key features and details
- Rabbit polyclonal to Synapsin I - Synaptic Marker
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Rat, Sheep, Goat
- Isotype: IgG
Overview
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Product name
Anti-Synapsin I antibody - Synaptic Marker
See all Synapsin I primary antibodies -
Description
Rabbit polyclonal to Synapsin I - Synaptic Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Goat
Predicted to work with: Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Rat Synapsin I aa 600-700 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
(Peptide available asab64580) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab64581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa). Abcam recommends using 3-5% milk as the blocking agent. |
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| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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Function
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
Involvement in disease
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
Sequence similarities
Belongs to the synapsin family. -
Post-translational
modificationsSubstrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell junction > synapse. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
- SwissProt: P09951 Rat
- Unigene: 225936 Human
see all -
Alternative names
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
Images
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Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image is courtesy of an anonymous AbreviewAnti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution + Rat whole brain tissue lysate at 15 µg
Secondary
Donkey anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 80,85 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocked with 5% milk for 1 hour at 25°C.
Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image was taken from an abreview submitted by an Carl Hobbs.IHC-P image of ab64581 staining of Synapsin 1 in lamb cerebellum. Heat mediated antigen retrieval buffer was performed using citric acid buffer. The sections were then incubated in 1% BSA to block non-specific protein-protein interactions. The sections were incubated with ab64581 (1:100) for two hours at +21°C, followed by biotin conjugated secondary antibody. There appears to be appropriate, identical positivity in lamb, goat, mouse and rat brain. -
Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)All lanes : Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?
The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data). -
Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.
Protocols
References (43)
ab64581 has been referenced in 43 publications.
- Sharma Y et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
- Nie PY et al. miR-132 Regulates PTSD-like Behaviors in Rats Following Single-Prolonged Stress Through Fragile X-Related Protein 1. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 32333305
- Xu XE et al. Caspase-1 inhibitor exerts brain-protective effects against sepsis-associated encephalopathy and cognitive impairments in a mouse model of sepsis. Brain Behav Immun 80:859-870 (2019). PubMed: 31145977
- Liu P et al. Constraint induced movement therapy promotes contralesional-oriented structural and bihemispheric functional neuroplasticity after stroke. Brain Res Bull 150:201-206 (2019). PubMed: 31181321
- Ackermann F et al. Critical role for Piccolo in synaptic vesicle retrieval. Elife 8:N/A (2019). PubMed: 31074746
