Anti-Synapsin I antibody - Synaptic Marker (ab64581)

Reviews (14)Q&A (4)References (43)

Key features and details

  • Rabbit polyclonal to Synapsin I - Synaptic Marker
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Sheep, Goat
  • Isotype: IgG

Overview

  • Product name

    Anti-Synapsin I antibody - Synaptic Marker
    See all Synapsin I primary antibodies

  • Description

    Rabbit polyclonal to Synapsin I - Synaptic Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Goat
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Rat Synapsin I aa 600-700 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab64580)

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa).

Abcam recommends using 3-5% milk as the blocking agent.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • Function

    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

  • Involvement in disease

    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

  • Sequence similarities

    Belongs to the synapsin family.

  • Post-translational
    modifications

    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

Images

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image is courtesy of an anonymous Abreview
    Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution + Rat whole brain tissue lysate at 15 µg

    Secondary
    Donkey anti-Rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 80,85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocked with 5% milk for 1 hour at 25°C.

    Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image was taken from an abreview submitted by an Carl Hobbs.
    IHC-P image of ab64581 staining of Synapsin 1 in lamb cerebellum. Heat mediated antigen retrieval buffer was performed using citric acid buffer. The sections were then incubated in 1% BSA to block non-specific protein-protein interactions. The sections were incubated with ab64581 (1:100) for two hours at +21°C, followed by biotin conjugated secondary antibody. There appears to be appropriate, identical positivity in lamb, goat, mouse and rat brain.

    See Abreview

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    All lanes : Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 70,74 kDa why is the actual band size different from the predicted?



    The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data).
  • Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.

References (43)

Publishing research using ab64581? Please let us know so that we can cite the reference in this datasheet.

ab64581 has been referenced in 43 publications.

  • Sharma Y  et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
  • Nie PY  et al. miR-132 Regulates PTSD-like Behaviors in Rats Following Single-Prolonged Stress Through Fragile X-Related Protein 1. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 32333305
  • Xu XE  et al. Caspase-1 inhibitor exerts brain-protective effects against sepsis-associated encephalopathy and cognitive impairments in a mouse model of sepsis. Brain Behav Immun 80:859-870 (2019). PubMed: 31145977
  • Liu P  et al. Constraint induced movement therapy promotes contralesional-oriented structural and bihemispheric functional neuroplasticity after stroke. Brain Res Bull 150:201-206 (2019). PubMed: 31181321
  • Ackermann F  et al. Critical role for Piccolo in synaptic vesicle retrieval. Elife 8:N/A (2019). PubMed: 31074746
View all Publications for this product

Customer reviews and Q&As

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1-10 of 18 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
None
Permeabilization
Yes - PBS 1x, Triton X-100 1%
Specification
Cerebellum
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 04 2020

Western blot abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Cerebellum)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
20 µg
Specification
Cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 27 2020

Western blot abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Whole Brain)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
15 µg
Specification
Whole Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 01 2015

Question

I would like to enroll in your AbTrial Program. I will be using your antibodies for immunohistochemical experiments with Rhesus monkey brain tissue.

We will be examining synaptic markers inbrain tissue of rhesus macaques previously exposed to SIV.

Since your antibodies do not say that they work in rhesus monkey, I am requesting a trial for the testing of the following antibodies:

anti-synaptophysin (ab8049)

anti-hypophosphorylated neurofilament (ab82259)

antisynapsin (ab64581)

anti-PSD95 (ab18258)

anti-cathepsin B (ab58802)

anti-IBA1 (ab5076)

anti-GFAP (ab4674)

I have just placed a purchase order for these antibodies.

Read More
Answer

I am very pleased to hear you would like to test ab5076, ab8049, ab18258, ab58802, ab64581, ab82259 and send us your results. The testing discount doesn't apply to ab4674 since this has already been tested in Rhesus monkey and IHC so we would guarantee that experiment to work.

The codes will give you a value off your next orders before the expiration date. To redeem this offer, please submit 6 Abreview with rhesus monkey data and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview wit has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain-Cerebellum)
Specification
Brain-Cerebellum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
Yes - 0.01% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jul 28 2012

Question

i am a researcher who bought one of abcam product for detecting synapsin 1 in brain of rat.the catnumber of antibody is ab59268. i had chosen ab97199 FITC as secondary antibody which was recommended as compatible secondary. i faced a problem about the pattern of immunoreactivity on my slides. seems that the reaction between the primary and secondary is all right but i can just see immunoreactivity around the cells instead of along the axons. i was expecting a pattern of immunoreactivity like the picture which is on the page of ab64581.
whould it be possible for you to tell me why the pattern of my immunoreactivity is not along the axons?
i am looking forward to getting your consultation.

Read More
Answer

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody/antibodies.
As I am not quite sure if you have performed ICC or IHC I have attached the questionnaire twice.
I look forward to receiving your reply.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain coronal sections)
Specification
Brain coronal sections
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-sodium citrate buffer
Permeabilization
Yes - 0.2 % triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Feb 24 2012

Question

Thank you very much for the syn1 antibody, it works great on brain sections!!!
I will be happy to post a (nice) abreview :-)

Read More
Answer

That's great to hear! I'd really appreciate it if you would take the time to submit an Abreview - this should be helpful for other customers.

Have a wonderful day, and please don't hesitate to let me know if there is anything else I can help with!

Read More

Immunocytochemistry/ Immunofluorescence abreview for Anti-Synapsin I antibody - Synaptic Marker

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal neuron culture)
Specification
Hippocampal neuron culture
Fixative
Methanol
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Read More

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 09 2012

Question

Recently, I've been doing some experiments with some colleagues on synapsin 1.
I have purchased 2 antibodies from abcam: one N-ter (ab18814) for WB and one (ab8) for IF.

The ab18814 is working great! Do you think you could tell me the amino acids recognized by this antibody? my colleagues have performed MALDI technique and they need to make sure that the peptide captured by proteomic is the same than the one seen in WB.

For IF, unfortunately, the ab8 gave poor results on mouse fixed brain sections, with small numbers of neurons stained (ab18814 didn't work on IF neither).
Do you have any recommendations for another ab to test? Maybe ab64581?

Read More
Answer

Regarding ab8, I'm very sorry to hear that it isn't working for you! This should be working in IHC on mouse tissues, so I'm not sure why it is giving you trouble. I took a look through our catalog, and it looks like ab64581 would be a good replacement. Ab64581 has been tested in IHC and reacts with mouse, and has very positive reviews. I've included a link to the datasheet below so you can take a look and make sure this is the one you want. I'm happy to send you a vial free of charge.

For ab18814, this product is produced by a third party so we do not have the exact immunogen sequence. We do know that the immunogen peptide is taken from around amino acid 12. In my experience, immunogen peptides are normallysomewhere around 15-20 amino acids long, but obviously this could be different for this antibody. I'm going to see how much information I can get from the originator of the antibody, but I am not sure whether they will release the exact sequence to us.

Please let me know if you'd like a vial of ab64581 and I will set the order up for you.

I look forward to your reply. Have a great day!

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1-10 of 18 Abreviews or Q&A

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