Key features and details
- Rabbit polyclonal to Synapsin I (phospho S549)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Synapsin I (phospho S549) antibody
See all Synapsin I primary antibodies
DescriptionRabbit polyclonal to Synapsin I (phospho S549)
Specificityab119370 detects endogenous levels of Synapsin I only when phosphorylated at Serine 549.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a sequence surrounding phosphorylation site Serine 549 (PASPPS) of Rat Synapsin I (P09951).
- Rat and Mouse Brain tissue.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non phosphospecific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab119370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 74 kDa. Incubate membrane with diluted antibody in 5% non-fat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.|
FunctionNeuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
Involvement in diseaseDefects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
Sequence similaritiesBelongs to the synapsin family.
modificationsSubstrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell junction > synapse. Golgi apparatus.
- Information by UniProt
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
ab119370 has been referenced in 3 publications.
- Yu G et al. Cystatin C promotes cognitive dysfunction in rats with cerebral microbleeds by inhibiting the ERK/synapsin Ia/Ib pathway. Exp Ther Med 19:2282-2290 (2020). PubMed: 32104295
- Fu T et al. Modulation of cPKC? on Synapsin-Ia/b-Specific Phosphorylation Sites in the Developing Visual Cortex of Mice. Invest Ophthalmol Vis Sci 60:2676-2684 (2019). PubMed: 31242289
- Zhang N et al. cPKC? alleviates ischemic injury through modulating synapsin Ia/b phosphorylation in neurons of mice. Brain Res Bull 142:156-162 (2018). PubMed: 30016727