Overview

  • Product name

    Anti-Synapsin I (phospho S549) antibody
    See all Synapsin I primary antibodies
  • Description

    Rabbit polyclonal to Synapsin I (phospho S549)
  • Host species

    Rabbit
  • Specificity

    ab119370 detects endogenous levels of Synapsin I only when phosphorylated at Serine 549.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a sequence surrounding phosphorylation site Serine 549 (PASPPS) of Rat Synapsin I (P09951).

  • Positive control

    • Rat and Mouse Brain tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non phosphospecific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 74 kDa. Incubate membrane with diluted antibody in 5% non-fat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.

Target

  • Function

    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
  • Involvement in disease

    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.
  • Sequence similarities

    Belongs to the synapsin family.
  • Post-translational
    modifications

    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

Images

  • All lanes : Anti-Synapsin I (phospho S549) antibody (ab119370) at 1/1000 dilution

    Lane 1 : Rat Brain lysate.
    Lane 2 : Mouse Brain lysate.

    Predicted band size: 74 kDa

References

This product has been referenced in:

  • Zhang N  et al. cPKC? alleviates ischemic injury through modulating synapsin Ia/b phosphorylation in neurons of mice. Brain Res Bull 142:156-162 (2018). Read more (PubMed: 30016727) »
See 1 Publication for this product

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