Recombinant Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
![Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)](https://www.abcam.com/ps/products/32/ab32532/Images/ab32532-367039-anti-synapsin-I-phospho-s553-antibody-e377-westernblot-brain-human.jpg "Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E377] to Synapsin I (phospho S553)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Synapsin I (phospho S553) antibody [E377]
See all Synapsin I primary antibodies -
Description
Rabbit monoclonal [E377] to Synapsin I (phospho S553) -
Host species
Rabbit -
Specificity
This antibody only detects Synapsin phosphorylated on Serine 553 -
Tested Applications & Species
See all applications and species dataApplication Species Dot HumanWB MouseRatHuman
-
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser553 of Synapsin (Human)
-
Positive control
- WB: Untreated Human fetal brain, Mouse brain lysates, and Human fetal brain treated with alkaline phosphatase lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E377 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Dot |
Human
|
| WB |
Mouse
Rat
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
|
|
| Dot blot |
1/500.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
|
Dot blot
1/500. |
Target
-
Function
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. -
Involvement in disease
Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior. -
Sequence similarities
Belongs to the synapsin family. -
Post-translational
modificationsSubstrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell junction > synapse. Golgi apparatus. - Information by UniProt
-
Database links
- Entrez Gene: 6853 Human
- Entrez Gene: 20964 Mouse
- Entrez Gene: 24949 Rat
- Omim: 313440 Human
- SwissProt: P17600 Human
- SwissProt: O88935 Mouse
- SwissProt: P09951 Rat
- Unigene: 225936 Human
see all -
Alternative names
- Brain protein 4.1 antibody
- SYN 1 antibody
- SYN 1a antibody
see all
Images
-
Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution (Purified)
Lane 1 : Untreated Human fetal brain lysates
Lane 2 : Human fetal brain lysates treated with alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)](https://www.abcam.com/ps/products/32/ab32532/Images/ab32532-255949-anti-synapsin-i-phospho-s553-antibody-e377-western-blot.jpg)
Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution
Lane 1 : Untreated rat brain whole cell lysates
Lane 2 : Rat brain whole cell lysates, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBST
-
![Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)](https://www.abcam.com/ps/products/32/ab32532/Images/ab32532-255946-anti-synapsin-i-phospho-s553-antibody-e377-western-blot.jpg)
Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution
Lane 1 : Untreated mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysate, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
-
![Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)](https://www.abcam.com/ps/products/32/ab32532/Images/ab32532-255939-anti-synapsin-i-phospho-s553-antibody-e377-western-blot.jpg)
Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution
Lane 1 : Untreated mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysates, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBST
-
![Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)](https://www.abcam.com/ps/products/32/ab32532/Images/ab32532-45124-anti-synapsin-i-phospho-s553-antibody-e377-dot-blot.jpg)
Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)Dot Blot analysis on human immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500
-
![Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)](https://www.abcam.com/ps/products/32/ab32532/Images/ab32532-7-benefits-of-recombinant-antibodies.png)
Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab32532 has been referenced in 3 publications.
- Liu W et al. Aldehyde dehydrogenase 2 activation ameliorates cyclophosphamide-induced acute cardiotoxicity via detoxification of toxic aldehydes and suppression of cardiac cell death. J Mol Cell Cardiol 121:134-144 (2018). PubMed: 29981795
- Verstegen AM et al. Phosphorylation of synapsin I by cyclin-dependent kinase-5 sets the ratio between the resting and recycling pools of synaptic vesicles at hippocampal synapses. J Neurosci 34:7266-80 (2014). PubMed: 24849359
- Sriram K et al. Dopaminergic neurotoxicity following pulmonary exposure to manganese-containing welding fumes. Arch Toxicol 84:521-40 (2010). WB ; Rat . PubMed: 20224926