Recombinant
RabMAb

Recombinant Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-Synapsin I (phospho S553) antibody [E377]
    See all Synapsin I primary antibodies

  • Description

    Rabbit monoclonal [E377] to Synapsin I (phospho S553)

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Synapsin phosphorylated on Serine 553

  • Tested applications

    Suitable for: WB, Dot blotmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Ser553 of Synapsin (Human)

  • Positive control

    • WB: Untreated Human fetal brain, Mouse brain lysates, and Human fetal brain treated with alkaline phosphatase lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab32532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
Dot blot 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC or IP.

    • Target

    • Function

      Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

    • Involvement in disease

      Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

    • Sequence similarities

      Belongs to the synapsin family.

    • Post-translational
      modifications

      Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Cell junction > synapse. Golgi apparatus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Brain protein 4.1 antibody
      • SYN 1 antibody
      • SYN 1a antibody
      • SYN 1b antibody
      • SYN I antibody
      • SYN1 antibody
      • SYN1_HUMAN antibody
      • SYN1a antibody
      • SYN1b antibody
      • Synapsin 1 antibody
      • Synapsin I antibody
      • Synapsin-1 antibody
      • Synapsin1 antibody
      • SynapsinI antibody
      • SYNI antibody
      see all

    Images

    • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution (Purified)

      Lane 1 : Untreated Human fetal brain lysates
      Lane 2 : Human fetal brain lysates treated with alkaline phosphatase

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 74 kDa
      Observed band size: 77 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

      Lane 1 : Untreated rat brain whole cell lysates
      Lane 2 : Rat brain whole cell lysates, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 74 kDa
      Observed band size: 77 kDa why is the actual band size different from the predicted?


      Exposure time: 1 second


      Blocking buffer: 2% BSA/TBST

      Dilution buffer: 2% BSA/TBST

    • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

      Lane 1 : Untreated mouse brain whole cell lysate
      Lane 2 : Mouse brain whole cell lysate, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 74 kDa
      Observed band size: 77 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution

      Lane 1 : Untreated mouse brain whole cell lysate
      Lane 2 : Mouse brain whole cell lysates, treated with alkaline phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 74 kDa
      Observed band size: 77 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking buffer: 2% BSA/TBST

      Dilution buffer: 2% BSA/TBST

    • Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
      Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500

    References

    This product has been referenced in:

    • Liu W  et al. Aldehyde dehydrogenase 2 activation ameliorates cyclophosphamide-induced acute cardiotoxicity via detoxification of toxic aldehydes and suppression of cardiac cell death. J Mol Cell Cardiol 121:134-144 (2018). Read more (PubMed: 29981795) »
    • Verstegen AM  et al. Phosphorylation of synapsin I by cyclin-dependent kinase-5 sets the ratio between the resting and recycling pools of synaptic vesicles at hippocampal synapses. J Neurosci 34:7266-80 (2014). Read more (PubMed: 24849359) »
    See all 3 Publications for this product

    Publishing research using ab32532? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I am interested in staining synapses in a neuronal culture and I am trying to decide whether to use Synapsin or Synaptophysin antibodies. Do you have any suggestions about either or where to go from here? thanks.

    Read More
    Answer

    Thank you for contacting us and sharing your needs with us. Unfortunately, we are not specialists in this area and I do not want to recommend a product that may be unsuitable for your needs. May I please suggest that you refer to the latest literature in this field, I hope it will be useful and provide good information on what scientists are currently using as markers. I'm sorry I cannot help you more on this occasion. If you have questions on any of our antibodies I would be happy to help,

    Read More

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