Product nameAnti-Synapsin II antibody
See all Synapsin II primary antibodies
DescriptionRabbit polyclonal to Synapsin II
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- Rat brain tissue extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab13258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 74, 54 kDa (predicted molecular weight: 57.5 kDa).|
|ICC/IF||Use at an assay dependent dilution. PubMed: 19714568|
RelevanceSynapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal.
Cellular localizationCell junction, synapse.
- SYN 2 antibody
- SYN II antibody
- SYN IIa antibody
Anti-Synapsin II antibody (ab13258) at 1/2000 dilution + Rat whole brain tissue lysate at 15 µg
HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57.5 kDa
Observed band size: 60,78 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
Blocked with 5% milk for 1 hour at 25°C.
Incubated with the primary antibody diluted in TBS + 0.1% Tween for 18 hours at 4°C.
Western blot analysis of rat cerebellum tissue, staining Synapsin II with ab13258.
Samples were immunoprecipitated with antibodies against CB or mGluR4 and co-immunoprecipitated Synapsin II was detected using ab13258. Synapsin I was detected using ab8 - Rabbit polyclonal to Synapsin I.
This product has been referenced in:
- Shi W et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). IF . Read more (PubMed: 27592818) »
- Ramos C et al. Native presynaptic metabotropic glutamate receptor 4 (mGluR4) interacts with exocytosis proteins in rat cerebellum. J Biol Chem 287:20176-86 (2012). WB ; Rat . Read more (PubMed: 22528491) »