Recombinant Anti-SYN2 antibody [EPR3277] (ab76494)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3277] to SYN2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SYN2 antibody [EPR3277]
See all SYN2 primary antibodies -
Description
Rabbit monoclonal [EPR3277] to SYN2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SYN2 aa 500-600. The exact sequence is proprietary.
(Peptide available asab177842) -
Positive control
- WB: fetal brain cell lysate; IHC-P: human brain tissue;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3277 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000 - 1/20000. Predicted molecular weight: 63 kDa.Can be blocked with SYN2 peptide (ab177842). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons. -
Tissue specificity
Central and peripheral nervous systems. -
Sequence similarities
Belongs to the synapsin family. -
Domain
The A region binds phospholipids with a preference for negatively charged species. -
Post-translational
modificationsPhosphorylation at Ser-10 dissociates synapsins from synaptic vesicles. Phosphorylation at Ser-425 by MAPK1/ERK2 and/or MAPK3/ERK1 may play a role in noradrenaline secretion by sympathetic neurons. -
Cellular localization
Cell junction, synapse. - Information by UniProt
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Database links
- Entrez Gene: 6854 Human
- Entrez Gene: 20965 Mouse
- Entrez Gene: 29179 Rat
- Omim: 600755 Human
- SwissProt: Q92777 Human
- SwissProt: Q64332 Mouse
- SwissProt: Q63537 Rat
- Unigene: 445503 Human
see all -
Alternative names
- SYN 2 antibody
- SYN II antibody
- SYN IIa antibody
see all
Images
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Anti-SYN2 antibody [EPR3277] (ab76494) at 1/20000 dilution + Fetal brain tissue lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded human brain tissue using 1/100 ab76494
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab76494 has been referenced in 11 publications.
- Sakai Y et al. Gene-environment interactions mediate stress susceptibility and resilience through the CaMKIIß/TARP?-8/AMPAR pathway. iScience 24:102504 (2021). PubMed: 34113835
- Li S et al. The UL16 protein of HSV-1 promotes the metabolism of cell mitochondria by binding to ANT2 protein. Sci Rep 11:14001 (2021). PubMed: 34234233
- Nie L et al. Loganin substantially ameliorates molecular deficits, pathologies and cognitive impairment in a mouse model of Alzheimer's disease. Aging (Albany NY) 13:23739-23756 (2021). PubMed: 34689137
- Li Y et al. Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins. Oxid Med Cell Longev 2020:5408452 (2020). PubMed: 32587661
- Kim JH et al. Decreased dopamine in striatum and difficult locomotor recovery from MPTP insult after exposure to radiofrequency electromagnetic fields. Sci Rep 9:1201 (2019). PubMed: 30718744