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    synaptobrevin-antibody-sp10-ab18013.pdf

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Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs
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Anti-Synaptobrevin antibody [SP10] (ab18013)

  • Datasheet
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Key features and details

  • Mouse monoclonal [SP10] to Synaptobrevin
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey
  • Isotype: IgM

Get better batch-to-batch reproducibility with a recombinant antibody

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Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Synaptobrevin antibody [SP10]
  • Description

    Mouse monoclonal [SP10] to Synaptobrevin
  • Host species

    Mouse
  • Specificity

    Detects a ~18kD protein in WB, corresponding to predicted size of VAMP from human, mouse, rat, bovine, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus tissue. This antibody detects VAMP1 (amino acids 1-118) and VAMP2 (amino acids 33-96)-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. The clone number has been updated from (4E240) to (SP10 ) both clone numbers name the same antibody clone.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Tissue/ cell preparation (Human). Mice were immunized with synaptic vesicle containing fractions which were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies.

  • Positive control

    • Rat brain.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Constituents: PBS, 0.00174% PMSF, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    SP10
  • Isotype

    IgM
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration.

Target

  • Relevance

    Synaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
  • Cellular localization

    Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane.
  • Database links

    • Entrez Gene: 6843 Human
    • Entrez Gene: 22317 Mouse
    • Entrez Gene: 100519841 Pig
    • Entrez Gene: 25624 Rat
    • Omim: 185880 Human
    • SwissProt: P23763 Human
    • SwissProt: Q62442 Mouse
    • SwissProt: Q63666 Rat
    • Alternative names

      • SYB antibody
      • SYB1 antibody
      • Synaptobrevin 1 antibody
      • VAMP antibody
      • VAMP1 antibody
      • Vesicle associated membrane protein 1 antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab18013? Please let us know so that we can cite the reference in this datasheet.

    ab18013 has been referenced in 2 publications.

    • Lu CW  et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
    • Schilling JM  et al. Inhibition of p75 neurotrophin receptor does not rescue cognitive impairment in adulthood after isoflurane exposure in neonatal mice. Br J Anaesth 119:465-471 (2017). PubMed: 28969308

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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