Anti-Synaptobrevin antibody [SP10] (ab18013)
Key features and details
- Mouse monoclonal [SP10] to Synaptobrevin
- Suitable for: WB
- Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey
- Isotype: IgM
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Overview
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Product name
Anti-Synaptobrevin antibody [SP10] -
Description
Mouse monoclonal [SP10] to Synaptobrevin -
Host species
Mouse -
Specificity
Detects a ~18kD protein in WB, corresponding to predicted size of VAMP from human, mouse, rat, bovine, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus tissue. This antibody detects VAMP1 (amino acids 1-118) and VAMP2 (amino acids 33-96)-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. The clone number has been updated from (4E240) to (SP10 ) both clone numbers name the same antibody clone. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey -
Immunogen
Tissue/ cell preparation (Human). Mice were immunized with synaptic vesicle containing fractions which were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies.
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Positive control
- Rat brain.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituents: PBS, 0.00174% PMSF, 50% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
SP10 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. |
Target
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Relevance
Synaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. -
Cellular localization
Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane. -
Database links
- Entrez Gene: 6843 Human
- Entrez Gene: 22317 Mouse
- Entrez Gene: 100519841 Pig
- Entrez Gene: 25624 Rat
- Omim: 185880 Human
- SwissProt: P23763 Human
- SwissProt: Q62442 Mouse
- SwissProt: Q63666 Rat
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Alternative names
- SYB antibody
- SYB1 antibody
- Synaptobrevin 1 antibody
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Datasheets and documents
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Datasheet download
References (2)
ab18013 has been referenced in 2 publications.
- Lu CW et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
- Schilling JM et al. Inhibition of p75 neurotrophin receptor does not rescue cognitive impairment in adulthood after isoflurane exposure in neonatal mice. Br J Anaesth 119:465-471 (2017). PubMed: 28969308
