Anti-Synaptobrevin antibody [SP10] (ab18013)

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Overview

  • Product name
    Anti-Synaptobrevin antibody [SP10]
    See all Synaptobrevin primary antibodies
  • Description
    Mouse monoclonal [SP10] to Synaptobrevin
  • Host species
    Mouse
  • Specificity
    Detects a ~18kD protein in WB, corresponding to predicted size of VAMP from human, mouse, rat, bovine, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus tissue. This antibody detects VAMP1 (amino acids 1-118) and VAMP2 (amino acids 33-96)-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. The clone number has been updated from (4E240) to (SP10 ) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey
  • Immunogen

    Tissue/ cell preparation (Human). Mice were immunized with synaptic vesicle containing fractions which were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies.

  • Positive control
    • Rat brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab18013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 13 kDa.

Target

  • Relevance
    Synaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
  • Cellular localization
    Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane.
  • Database links
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  • Alternative names
    • SYB antibody
    • SYB1 antibody
    • Synaptobrevin 1 antibody
    • VAMP antibody
    • VAMP1 antibody
    • Vesicle associated membrane protein 1 antibody
    see all

References

ab18013 has not yet been referenced specifically in any publications.

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