Key features and details
- Mouse monoclonal [SP10] to Synaptobrevin
- Reacts with: Mouse, Rat, Hamster, Cow, Pig, Xenopus laevis
- Isotype: IgM
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Product nameAnti-Synaptobrevin antibody [SP10]
DescriptionMouse monoclonal [SP10] to Synaptobrevin
SpecificityDetects a ~18kD protein in WB, corresponding to predicted size of VAMP from human, mouse, rat, bovine, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus tissue. This antibody detects VAMP1 (amino acids 1-118) and VAMP2 (amino acids 33-96)-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. The clone number has been updated from (4E240) to (SP10 ) both clone numbers name the same antibody clone.
Species reactivityReacts with: Mouse, Rat, Hamster, Cow, Pig, Xenopus laevis
Tissue/ cell preparation (Human). Mice were immunized with synaptic vesicle containing fractions which were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies.
- Rat brain.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Constituents: PBS, 0.00174% PMSF, 50% Glycerol
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
RelevanceSynaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
Cellular localizationCytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane.
- SYB antibody
- SYB1 antibody
- Synaptobrevin 1 antibody
ab18013 has been referenced in 1 publication.
- Schilling JM et al. Inhibition of p75 neurotrophin receptor does not rescue cognitive impairment in adulthood after isoflurane exposure in neonatal mice. Br J Anaesth 119:465-471 (2017). PubMed: 28969308
Customer reviews and Q&As
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