Anti-Synaptobrevin antibody [SP10] (ab18013)
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Overview
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Product nameAnti-Synaptobrevin antibody [SP10]
See all Synaptobrevin primary antibodies -
DescriptionMouse monoclonal [SP10] to Synaptobrevin
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Host speciesMouse
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SpecificityDetects a ~18kD protein in WB, corresponding to predicted size of VAMP from human, mouse, rat, bovine, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus tissue. This antibody detects VAMP1 (amino acids 1-118) and VAMP2 (amino acids 33-96)-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. The clone number has been updated from (4E240) to (SP10 ) both clone numbers name the same antibody clone.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Human, Pig, Xenopus laevis, Monkey
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Immunogen
Tissue/ cell preparation (Human). Mice were immunized with synaptic vesicle containing fractions which were immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies.
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Positive control
- Rat brain.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Constituents: PBS, 0.00174% PMSF, 50% Glycerol -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone numberSP10
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IsotypeIgM
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab18013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 13 kDa. |
Target
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RelevanceSynaptobrevins are highly conserved between vertebrates and invertebrates. It is a major constituent of synaptic vesicles and peptidergic secretory granules in all neurons examined so far. In addition, it is present on secretory granules of neuroendocrine cells. Synaptobrevins, Syntaxins, and the 25 kDa synaptosomal associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.
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Cellular localizationCytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Single-pass type IV membrane protein. Cell junction > synapse > synaptosome. Isoform 3 is found in mitochondria, mitochondrial outer membrane.
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Database links
- Entrez Gene: 6843 Human
- Entrez Gene: 22317 Mouse
- Entrez Gene: 100519841 Pig
- Entrez Gene: 25624 Rat
- Omim: 185880 Human
- SwissProt: P23763 Human
- SwissProt: Q62442 Mouse
- SwissProt: Q63666 Rat
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Alternative names
- SYB antibody
- SYB1 antibody
- Synaptobrevin 1 antibody
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Protocols
Datasheets and documents
References
ab18013 has not yet been referenced specifically in any publications.
